Treatment | Carqueja hydroalcoholic extract | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | pharyngeal pumping | | | | locomotion on solid media | | | | body length / growth rate | | | | number of offspring | | | | vertex (progeny per day) | | | | oxidative stress resistance | | | | proteasomal activity | | | | ROS level | | | | activity / quantity / expression of HSPs | | | | activity / quantity / expression of HSPs | | | | triglyceride / fat / lipid content | | | | activity / quantity / expression of SODs | | | | activity / quantity / expression of GCS-1 | | | | activity / quantity / expression of GSTs | | | | median lifespan | | | | mean lifespan | | | | maximum lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
0.5 mg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | down | -7.84 | no | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
5 mg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | down | -2.21 | no | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | none | | no | L4 | | | | | none | | no | L4 | none | | no | | none | | no | | up | 27.08 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | up | | yes | L4 | down | | yes | L4; measured via H2DCF-DA at 37°C; similar change when pre-exposed to 5 mM H2O2 | | | | | | | | | down | | yes | L4; measured via Nile Red | | | | | | | | | | | | | | | | | | | | | | | | | | | | HSP-16.2 and HSP-4 | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
0.5 mg/ml | whole life; start: L1 | OP50 | NGM agar | Kanamycin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 1.75 | no | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
5 mg/ml | whole life; start: L1 | OP50 | NGM agar | Kanamycin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | down | -16.94 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | Kanamycin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 8.96 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | HT115 (DE3) | NGM agar | ampicillin, IPTG | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 7.75 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | HT115 (DE3) with RNAi-vector against skn-1 & daf-16 | NGM agar | ampicillin, IPTG | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 28.08 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
0.5 mg/ml | whole life; start: L1 | OP50 | NGM agar | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 9.09 | n.d. | | up | 5.04 | no | | up | 1.10 | n.d. | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
5 mg/ml | whole life; start: L1 | OP50 | NGM agar | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 18.18 | n.d. | | down | -0.08 | no | | up | 7.73 | n.d. | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 18.18 | n.d. | | up | 0.92 | no | | up | 1.66 | n.d. | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 47.69 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 21.95 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | AU3 [nsy-1(ag3)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 15.79 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | Kanamycin | AU3 [nsy-1(ag3)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 13.63 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | KU4 [sek-1(km4)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 26.76 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | VC8 [jnk-1(gk7)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 10 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | Kanamycin | VC8 [jnk-1(gk7)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 6.27 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | AM1 [osr-1(rm1)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 15.48 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | KU25 [pmk-1(km25)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 19.18 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | MH37 [mpk-1(ku1); unc-32(e189)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 24 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | Kanamycin | MH37 [mpk-1(ku1); unc-32(e189)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 13.77 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | MT2605 [unc-43(n498n1186)] | 20°C | | | | | | | | | | | | | | | | | | | | | up | 22.35 | yes | L4; stressor: 7.5 mM tert-butyl hydrogen peroxide; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | LD1171 {ldIs3 [gcs-1p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | L4; expression of gcs-1; measured via fluorescence; pre-exposed to 7.5 mM tert-butyl hydrogen peroxide for 1 h; no significant change without pre-exposure | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
5 mg/ml | whole life; start: L1 | OP50 | NGM agar | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | no | L4; expression of gst-4; measured via fluorescence; pre-exposed to 7.5 mM tert-butyl hydrogen peroxide for 1 h; no change without pre-exposure | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | L4; expression of gst-4; measured via fluorescence; no significant change when pre-exposed to 7.5 mM tert-butyl hydrogen peroxide for 1 h | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | L4; expression of sod-3; measured via fluorescence; no significant change when pre-exposed to 7.5 mM tert-butyl hydrogen peroxide for 1 h | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | variable | | yes | L4; expression of hsp-16.2; measured via fluorescence; increased when pre-exposed to 35°C for 1 h; decreased without pre-exposure | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | SJ4005 {zcIs4 [hsp-4::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | L4; expression of hsp-16.2; measured via fluorescence; no change when pre-exposed to 35°C for 1 h | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 25°C | | | | | up | | yes | L3/L4; ratio of non-paralyzed and paralyzed nematodes | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |
50 mg/ml | whole life; start: L1 | OP50 | NGM agar | Kanamycin | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 25°C | | | | | up | | yes | L3/L4; ratio of non-paralyzed and paralyzed nematodes | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P (2015) Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. Oxid Med Cell Longev 2015:740162. | |