Treatment | Cranberry extract (Vaccinium macrocarpon) | cognitive function | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 135331993 | ChEMBL | | | | | memory | | | | locomotion on solid media | | | | number of offspring | | | | heat stress resistance | | | | oxidative stress resistance | | | | pathogen stress resistance | | | | osmotic stress resistance | | | | osmotic stress resistance | | | | UV stress resistance | | | | ROS level | | | | quantity of β-Amyloid | | | | translocation of DAF-16 to nucleus | | | | median lifespan | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
2 mg/ml | start: egg stage; stop: A2 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | none | | no | A2; endpoint: body bends per minute | | | | | up | | yes | A2; Stressor: 37 °C for 1.5 h; endpoint: mean lifespan | | | | | up | 11.50 | yes | A2; Stressor: Vibrio cholerae; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 12.80 | yes | Change of remaining lifespan after stop of treatment = 11.10 % | | | | | Guha S, Natarajan O, Murbach CG, Dinh J, Wilson EC, Cao M, Zou S, Dong Y (2014) Supplement timing of cranberry extract plays a key role in promoting Caenorhabditis elegans healthspan. Nutrients 6, 911-921. | |
2 mg/ml | start: egg stage; stop: A6 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | none | | no | A6; endpoint: body bends per minute | | | | | up | 61.30 | yes | A6; Stressor: 37 °C for 1.5 h; endpoint: mean lifespan | | | | | up | 23.30 | yes | A6; Stressor: Vibrio cholerae; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 14.40 | yes | Change of remaining lifespan after stop of treatment = 32.70 % | | | | | Guha S, Natarajan O, Murbach CG, Dinh J, Wilson EC, Cao M, Zou S, Dong Y (2014) Supplement timing of cranberry extract plays a key role in promoting Caenorhabditis elegans healthspan. Nutrients 6, 911-921. | |
2 mg/ml | start: egg stage; stop: A10 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | up | | yes | A10; endpoint: body bends per minute | | | | | up | 76.50 | yes | A10; Stressor: 37 °C for 1.5 h; endpoint: mean lifespan | | | | | up | 25.30 | yes | A10; Stressor: Vibrio cholerae; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 20.00 | yes | Change of remaining lifespan after stop of treatment = 80.80 % | | | | | Guha S, Natarajan O, Murbach CG, Dinh J, Wilson EC, Cao M, Zou S, Dong Y (2014) Supplement timing of cranberry extract plays a key role in promoting Caenorhabditis elegans healthspan. Nutrients 6, 911-921. | |
2 mg/ml | start: L4; stop: A2 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | down | | no | A2; endpoint: body bends per minute | | | | | up | | yes | A2; Stressor: 37 °C for 1.5 h; endpoint: mean lifespan | | | | | none | | no | A2; Stressor: Vibrio cholerae; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 8.20 | yes | percentage values correspond to the remaining lifespan after stop of treatment | | | | | Guha S, Natarajan O, Murbach CG, Dinh J, Wilson EC, Cao M, Zou S, Dong Y (2014) Supplement timing of cranberry extract plays a key role in promoting Caenorhabditis elegans healthspan. Nutrients 6, 911-921. | |
2 mg/ml | start: L4; stop: A6 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | none | | no | A6; endpoint: body bends per minute | | | | | up | 9.30 | yes | A6; Stressor: 37 °C for 1.5 h; endpoint: mean lifespan | | | | | none | | no | A6; Stressor: Vibrio cholerae; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 21.20 | yes | percentage values correspond to the remaining lifespan after stop of treatment | | | | | Guha S, Natarajan O, Murbach CG, Dinh J, Wilson EC, Cao M, Zou S, Dong Y (2014) Supplement timing of cranberry extract plays a key role in promoting Caenorhabditis elegans healthspan. Nutrients 6, 911-921. | |
2 mg/ml | start: L4; stop: A10 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | none | | no | A10; endpoint: body bends per minute | | | | | up | 33.80 | yes | A10; Stressor: 37 °C for 1.5 h; endpoint: mean lifespan | | | | | none | | no | A10; Stressor: Vibrio cholerae; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 69.70 | yes | percentage values correspond to the remaining lifespan after stop of treatment | | | | | Guha S, Natarajan O, Murbach CG, Dinh J, Wilson EC, Cao M, Zou S, Dong Y (2014) Supplement timing of cranberry extract plays a key role in promoting Caenorhabditis elegans healthspan. Nutrients 6, 911-921. | |
0.2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 7.69 | n.d. | | up | 12.37 | no | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 25 | n.d. | | up | 25.60 | yes | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
10 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -20 | n.d. | | down | -12.67 | no | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: 2 generations before | OP50 | NGM agar | | N2 (wild type) | 25°C | | | | | down | -20.83 | no | L4/A1; endpoint: body bends per minute | | | | | up | | yes | L4/A1; stressor: 37°C for 3.5 h; endpoint: survival | none | | no | L4/A1; stressor: 5 mM paraquat; endpoint: survival | | | | | down | | yes | L4/A1; stressor: 300 or 500 mM NaCl for 10 min; endpoint: motility | down | | yes | L4/A1; stressor: 500 mM NaCl; endpoint: survival | none | | no | L4/A1; stressor: 0.05 J/cm2 for 20 s; endpoint: survival | none | | no | A1 and A4; pre-exposed to 5 mM paraquat | | | | | | | | | | | | | | | | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | | N2 (wild type) | 25°C | | | | | | | | | down | -14.19 | no | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | up | 16.22 | no | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | | N2 (wild type) | 16°C | | | | | | | | | down | -2.35 | no | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | GR1307 [daf-16(mgDf50)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | n.d. | | up | 05.04 | no | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | VC199 [sir-2.1(ok434)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 18.75 | n.d. | | up | 19.25 | yes | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | AM1 [osr-1(rm1)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | n.d. | | down | -6.06 | no | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | AU1 [sek-1(ag1)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 10.71 | n.d. | | up | 10.71 | no | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | MT2605 [unc-43(n498n1186)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 6.67 | n.d. | | up | 6.48 | no | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | CB1370 [daf-2(e1370)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 3.57 | n.d. | | up | 4.58 | no | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | VC8 [jnk-1(gk7)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 26.67 | n.d. | | up | 25.66 | yes | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: L4/A1 | OP50 | NGM agar | FUDR | TJ1052 [age-1(hx546)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | n.d. | | down | -2.89 | no | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | 1 h; start: young adults | OP50 | NGM agar | | GR1352 {daf-16(mgDf47); xrIs87 [daf-16(alpha)::GFP::daf-16B + rol-6(su1006)]} | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | young adults | | | | | | | | | | | | | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | whole life; start: unknown | OP50 | NGM agar | | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up-regulation of hsp-12.6, sod-3, and daf-16; down-regulation of hsp-4, hsp-16.1, and hsp-16.49 | | | daf-16, daf-2, age-1, osr-1, and unc-43 | Guha S, Cao M, Kane RM, Savino AM, Zou S, Dong Y (2013) The longevity effect of cranberry extract in Caenorhabditis elegans is modulated by daf-16 and osr-1. Age (Dordr) 35, 1559-1574. | |
2 mg/ml | from eggs - L3 | OP50 | NGM agar | | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 16°C | up | 13,4 | yes | L3; shifted to 25°C; attractant: OP50 | up | | n.d. | L4; 25°C exposure for 24h prior measurement; paralysis assay | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -53,5 | yes | L4; 25°C exposure for 24h prior measurement; Aβ expression determined via qRT-PCR; similar but weaker results via western blot | | | | | | | | | | | | | | | | | Guo, H., Dong, Y. Q., & Ye, B. P. (2016). Cranberry extract supplementation exerts preventive effects through alleviating Aβ toxicity in Caenorhabditis elegans model of Alzheimer's disease. Chin J Nat Med, 14(6), 427-433. doi:10.1016/S1875-5364(16)30039-5 | |
2 mg/ml | whole life; start: L3 | OP50 | NGM agar | | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 16°C | up | 6,6 | yes | L3; shifted to 25°C; attractant: OP50 | up | | n.d. | L4; 25°C exposure for 24h prior measurement; paralysis assay; very weak effect | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -37,4 | yes | L4; 25°C exposure for 24h prior measurement; Aβ expression determined via qRT-PCR; similar but weaker results via western blot | | | | | | | | | | | | | | | | | Guo, H., Dong, Y. Q., & Ye, B. P. (2016). Cranberry extract supplementation exerts preventive effects through alleviating Aβ toxicity in Caenorhabditis elegans model of Alzheimer's disease. Chin J Nat Med, 14(6), 427-433. doi:10.1016/S1875-5364(16)30039-5 | |
2 mg/ml | whole life; start: egg stage | OP50 | NGM agar | | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 16°C | up | 19,4 | yes | L3; shifted to 25°C; attractant: OP50 | up | | n.d. | L4; 25°C exposure for 24h prior measurement; paralysis assay | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -69,6 | yes | L4; 25°C exposure for 24h prior measurement; Aβ expression determined via qRT-PCR; similar but weaker results via western blot | | | | | | | | | | | | | | | | | Guo, H., Dong, Y. Q., & Ye, B. P. (2016). Cranberry extract supplementation exerts preventive effects through alleviating Aβ toxicity in Caenorhabditis elegans model of Alzheimer's disease. Chin J Nat Med, 14(6), 427-433. doi:10.1016/S1875-5364(16)30039-5 | |