Treatment | DMF (Dimethyl formamide) | cognitive function | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | |||||||||||||||||||||||||||||||||||||||||||||||||||
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CID (PubChem) | 6228 | ChEMBL | chemotaxis | pharyngeal pumping | locomotion on solid media | number of offspring | reproductive total span | onset of egg laying | heat stress resistance | oxidative stress resistance | oxidative stress resistance | activity / quantity / expression of HSPs | translocation of DAF-16 to nucleus | median lifespan | maximum lifespan | |||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
0.5 % | whole life; start: egg stage | OP50 | liquid S-medium | N2 (wild type) | 20°C | up | 37.50 | yes | up | 56.25 | n.d. | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||||
0.75 % | whole life; start: egg stage | OP50 | liquid S-medium | N2 (wild type) | 20°C | up | yes | A2 | none | no | daily measuremets; monitoring of intake of fluorescent beeds | down | -35.09 | yes | up | n.d. | delayed | n.d. | down | yes | A3; Stressor: 35°C for 19 h | down | no | A2; Stressor: 1 mM H2O2 for 12 h; endpoint: median lifespan | down | yes | A2; Stressor: 40 mM paraquat for 44 h; endpoint: median lifespan | up | 32.25 | yes | up | 54.68 | n.d. | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||
1 % | whole life; start: egg stage | OP50 | liquid S-medium | N2 (wild type) | 20°C | up | 33.33 | yes | up | 33.33 | n.d. | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||||
1.5 % | whole life; start: egg stage | OP50 | liquid S-medium | N2 (wild type) | 20°C | down | n.d. | down | n.d. | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.75 % | whole life; start: end of egg-laying period | OP50 | liquid S-medium | N2 (wild type) | 20°C | none | no | up | 25.71 | n.d. | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
0.75 % | whole life; start: egg stage | OP50 | liquid S-medium | DR1572 [daf-2(e1368)] | 20°C | down | n.d. | up | 45.00 | yes | up | 57.00 | n.d. | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||
0.75 % | whole life; start: egg stage | OP50 | liquid S-medium | TJ1052 [age-1(hx546)] | 20°C | down | n.d. | up | 59.00 | yes | up | 75.00 | n.d. | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||
0.75 % | whole life; start: egg stage | OP50 | liquid S-medium | DR26 [daf-16(m26)] | 20°C | up | 40.00 | yes | up | 43.00 | n.d. | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||||
0.75 % | whole life; start: egg stage | OP50 | liquid S-medium | CF1139[daf-16(mu86); muIs61 [(pKL78) daf16::GFP + rol-6(su1006)]] | 20°C | none | n.d. | measurements at several timepoints | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.75 % | whole life; start: egg stage | low-concentrated OP50 (DR conditions) | liquid S-medium | N2 (wild type) | 20°C | down | n.d. | delayed | n.d. | up | 53.00 | yes | up | 54.00 | n.d. | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||
0.75 % | 2 h; start: A2 | OP50 | liquid S-medium | N2 (wild type) | 20°C | up | yes | A2; Stressor: 1 mM H2O2 for 12 h; endpoint: median lifespan | none | no | A2; Stressor: 40 mM paraquat for 44 h; endpoint: median lifespan | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||||
0.75 % | whole life; start: egg stage | OP50 | liquid S-medium | CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]} | 20°C | none | n.d. | A3; measured via fluorescence | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.05 % | whole life; start: L3 | OP50 | NGM agar | CL4176 (dvIs27[myo-3::Ab3–42 let 39UTR(pAF29);pRF4 (rol-6(su1006))]) | 25°C | up | no | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 % | whole life; start: L3 | OP50 | NGM agar | CL4176 (dvIs27[myo-3::Ab3–42 let 39UTR(pAF29);pRF4 (rol-6(su1006))]) | 25°C | up | no | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.25 % | whole life; start: L3 | OP50 | NGM agar | CL4176 (dvIs27[myo-3::Ab3–42 let 39UTR(pAF29);pRF4 (rol-6(su1006))]) | 25°C | up | yes | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.5 % | whole life; start: L3 | OP50 | NGM agar | CL4176 (dvIs27[myo-3::Ab3–42 let 39UTR(pAF29);pRF4 (rol-6(su1006))]) | 25°C | up | yes | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 % | whole life; start: L3 | OP50 | NGM agar | CL4176 (dvIs27[myo-3::Ab3–42 let 39UTR(pAF29);pRF4 (rol-6(su1006))]) | 25°C | up | yes | Frankowski H, Alavez S, Spilman P, Mark KA, Nelson JD, Mollahan P, Rao RV, Chen SF, Lithgow GJ, Ellerby HM (2013) Dimethyl sulfoxide and dimethyl formamide increase lifespan of C. elegans in liquid. Mech Ageing Dev 134, 69-78. |