| Treatment | Diplocyclos palmatus, methanolic leaf extract | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CID (PubChem) | ChEMBL | pharyngeal pumping | pathogen stress resistance | UV stress resistance | ROS level | survival at a given time point | ||||||||||||||||||||||||||
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | ||||||
| 200 µg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | A1-A4 | up | n.d. | stressor: S. marcescens; medium: M9 buffer; endpoint: survival rate | up | yes | stressor: UV-A; medium: M9 buffer; endpoint: survival rate and pharyngeal pumping | down | yes | measurement via DCF-DA and after UV-A stress | down | no | tested in M9 buffer | upregulation of daf-16, daf-2; downregulation of age-1, eat-2 | Alexpandi R, Prasanth MI, Ravi AV, Balamurugan K, Durgadevi R, Srinivasan R, De Mesquita JF, Pandian SK (2019) Protective effect of neglected plant Diplocyclos palmatus on quorum sensing mediated infection of Serratia marcescens and UV-A induced photoaging in model Caenorhabditis elegans. J Photochem Photobiol B 201, 111637. | no | |||||||||
| 400 µg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | A1-A4 | up | n.d. | stressor: S. marcescens; medium: M9 buffer; endpoint: survival rate | down | no | tested in M9 buffer | upregulation of daf-16, daf-2; downregulation of age-1, eat-2 | Alexpandi R, Prasanth MI, Ravi AV, Balamurugan K, Durgadevi R, Srinivasan R, De Mesquita JF, Pandian SK (2019) Protective effect of neglected plant Diplocyclos palmatus on quorum sensing mediated infection of Serratia marcescens and UV-A induced photoaging in model Caenorhabditis elegans. J Photochem Photobiol B 201, 111637. | no | |||||||||||||||
| 600 µg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | A1-A4 | up | n.d. | stressor: S. marcescens; medium: M9 buffer; endpoint: survival rate | down | no | tested in M9 buffer | upregulation of daf-16; downregulation of age-1, eat-2 | Alexpandi R, Prasanth MI, Ravi AV, Balamurugan K, Durgadevi R, Srinivasan R, De Mesquita JF, Pandian SK (2019) Protective effect of neglected plant Diplocyclos palmatus on quorum sensing mediated infection of Serratia marcescens and UV-A induced photoaging in model Caenorhabditis elegans. J Photochem Photobiol B 201, 111637. | no | |||||||||||||||
| 800 µg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | tested in M9 buffer | Alexpandi R, Prasanth MI, Ravi AV, Balamurugan K, Durgadevi R, Srinivasan R, De Mesquita JF, Pandian SK (2019) Protective effect of neglected plant Diplocyclos palmatus on quorum sensing mediated infection of Serratia marcescens and UV-A induced photoaging in model Caenorhabditis elegans. J Photochem Photobiol B 201, 111637. | no | ||||||||||||||||||||||
| 1000 µg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | tested in M9 buffer | Alexpandi R, Prasanth MI, Ravi AV, Balamurugan K, Durgadevi R, Srinivasan R, De Mesquita JF, Pandian SK (2019) Protective effect of neglected plant Diplocyclos palmatus on quorum sensing mediated infection of Serratia marcescens and UV-A induced photoaging in model Caenorhabditis elegans. J Photochem Photobiol B 201, 111637. | no | ||||||||||||||||||||||