Treatment | Glochidion zeylanicum, methanol extract | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | pharyngeal pumping | | | | oxidative stress resistance | | | | ROS level | | | | activity / quantity / expression of HSPs | | | | translocation of DAF-16 to nucleus | | | | activity / quantity / expression of SODs | | | | translocation of SKN-1 to nucleus | | | | autofluorescence (age pigment / lipofuscin) | | | | activity / quantity / expression of GSTs | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | | | | | | |
1 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | N2 (wild type) | 20°C | up | | yes | A5, A8, A10, A12 | up | | yes | stressor: 80 μM juglone; endpoint: survival; 48h of compound treatment | down | -63.54 | yes | measured via H2DCF-DA; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | up | 7.98 | yes | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
2.5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | N2 (wild type) | 20°C | up | | yes | A5, A8, A10, A12; not significant at A12 | up | | yes | stressor: 80 μM juglone; endpoint: survival; 48h of compound treatment | down | -42.47 | yes | measured via H2DCF-DA; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | N2 (wild type) | 20°C | up | | yes | A5, A8, A10, A12; not significant at A12 | up | | no | stressor: 80 μM juglone; endpoint: survival; 48h of compound treatment | up | | no | measured via H2DCF-DA; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | up | 8.75 | yes | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
1 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | down | -67.09 | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
2.5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | down | -32.13 | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | down | -36.7 | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
1 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | up | 18.02 | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
2.5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | up | 16.2 | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | none | | no | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
1 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 29.8 | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
2.5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 57.4 | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 54.2 | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
1 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | up | | yes | 72h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
2.5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | up | | yes | 72h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | up | | yes | 72h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
1 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | 72h of compound treatment | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
2.5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | 72h of compound treatment | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | 72h of compound treatment | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
1 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -7.54 | yes | A16; blue fluorescence | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
2.5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -12.12 | yes | A16; blue fluorescence | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
5 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -8.17 | yes | A16; blue fluorescence | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |