| Treatment | Green rooibus extract (Aspalathus linearis) | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CID (PubChem) | ChEMBL | oxidative stress resistance | ROS level | activity / quantity / expression of HSPs | mean lifespan | |||||||||||||||||||||||
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
| 100 µg/ml | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | down | -30.60 | yes | A1; measurement via H2DCF-DA | none | no | DAF-16 | Chen W, Sudji IR, Wang E, Joubert E, van Wyk BE, Wink M (2013) Ameliorative effect of aspalathin from rooibos (Aspalathus linearis) on acute oxidative stress in Caenorhabditis elegans. Phytomedicine 20, 380-386. | |||||||||||||||
| 100 µg/ml | whole life; start: L1 | OP50 | NGM agar | 50 mM glucose | N2 (wild type) | 20°C | up | 22.50 | yes | Chen W, Sudji IR, Wang E, Joubert E, van Wyk BE, Wink M (2013) Ameliorative effect of aspalathin from rooibos (Aspalathus linearis) on acute oxidative stress in Caenorhabditis elegans. Phytomedicine 20, 380-386. | ||||||||||||||||||
| 100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | N2 (wild type) | 20°C | up | 106.00 | yes | A1; Stressor: 400 µM juglone for 6 h | Chen W, Sudji IR, Wang E, Joubert E, van Wyk BE, Wink M (2013) Ameliorative effect of aspalathin from rooibos (Aspalathus linearis) on acute oxidative stress in Caenorhabditis elegans. Phytomedicine 20, 380-386. | ||||||||||||||||||
| 100 µg/ml | start: L1; stop: L4 (48h) | OP50 | liquid S-medium | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | down | -26.70 | yes | A1; exposure to 20 µM juglone for 24 h before measurement; measurement via fluorescence | Chen W, Sudji IR, Wang E, Joubert E, van Wyk BE, Wink M (2013) Ameliorative effect of aspalathin from rooibos (Aspalathus linearis) on acute oxidative stress in Caenorhabditis elegans. Phytomedicine 20, 380-386. | ||||||||||||||||||