Treatment | Holothuria leucospilota, n-butanol extract | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | heat stress resistance | | | | translocation of DAF-16 to nucleus | | | | mean lifespan | | | | maximum lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | | | | | | |
10 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 25°C | | | | | | | | | up | 2.96 | no | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
100 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 25°C | | | | | | | | | up | 3.68 | yes | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
500 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 25°C | | | | | | | | | up | 5.92 | yes | | up | 13.33 | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
1000 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 25°C | | | | | | | | | down | -1.44 | no | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
10 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 25°C | | | | | | | | | up | 0.36 | no | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
100 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 25°C | | | | | | | | | up | 3.96 | yes | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
500 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 25°C | | | | | | | | | up | 15.76 | yes | | up | 23.5 | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
1000 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 25°C | | | | | | | | | up | 2.16 | no | | down | -5.9 | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
100 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | TJ1052 [age-1(hx546)] | 25°C | | | | | | | | | down | -1.92 | no | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
500 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | TJ1052 [age-1(hx546)] | 25°C | | | | | | | | | down | -0.99 | no | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
100 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | CF1038 [daf-16(mu86)] | 25°C | | | | | | | | | up | 3.11 | no | | up | 9 | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
500 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | CF1038 [daf-16(mu86)] | 25°C | | | | | | | | | up | 3.37 | no | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
100 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | VC199 [sir-2.1(ok434)] | 25°C | | | | | | | | | up | 2.78 | no | | up | 5.6 | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
500 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | VC199 [sir-2.1(ok434)] | 25°C | | | | | | | | | up | 1.72 | no | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
100 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | VC8 [jnk-1(gk7)] | 25°C | | | | | | | | | up | 03.03 | no | | up | 5.6 | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
500 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | VC8 [jnk-1(gk7)] | 25°C | | | | | | | | | up | 4.27 | no | | up | 5.6 | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
100 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 25°C | | | | | | | | | up | 7.89 | yes | | none | | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
500 µg/ml | whole life; start: L1 | OP50 | NGM agar | FUdR | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 25°C | | | | | | | | | up | 9.79 | yes | | up | 4.5 | n.d. | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
100 µg/ml | whole life; start: L1 | OP50 | NGM agar | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | | yes | A1, A4; stressor: 35°C for 4h; endpoint: survival | up | | yes | A1, A4 | | | | | | | | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |
500 µg/ml | whole life; start: L1 | OP50 | NGM agar | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | | yes | A1, A4; stressor: 35°C for 4h; endpoint: survival | up | | yes | A1, A4 | | | | | | | | | | | | | Kitisin T, Suphamungmee W, Meemon K (2019) Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16. J Food Biochem 43, e13075. | no |