1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | none | | no | A1; measured via H2DCF-DA | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
2 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | none | | no | A1; measured via H2DCF-DA | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
10 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | up | 22.6 | yes | A1; measured via H2DCF-DA | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |