Treatment | Liangyi Gao (TCM) | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||||||||||||||||
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CID (PubChem) | ChEMBL | pharyngeal pumping | locomotion on solid media | body length / growth rate | number of offspring | quantity of late reproduction | oxidative stress resistance | heat stress resistance | translocation of DAF-16 to nucleus | activity / quantity / expression of SODs | autofluorescence (age pigment / lipofuscin) | mean lifespan | ||||||||||||||||||||||||||||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | ||||||
1 mg/ml | whole life; start: n.d. | OP50 | NGM agar | N2 (wild type) | 20°C | up | n.d. | A4 | up | yes | A7 | up | yes | A4 | up | yes | up | n.d. | up | yes | A4; stressor: H2O2; endpoint: mean survival | up | yes | A4; stressor: 37°C; endpoint: mean survival | up | yes | down | -34.2 | yes | A10; green fluorescence | up | 23.8 | yes | upregulation of daf-16, sod-3, sir-2.1 | Zeng L, Sun C, Pei Z, Yun T, Fan S, Long S, Wu T, Chen Z, Yang Z, Xu F (2019) Liangyi Gao extends lifespan and exerts an antiaging effect in Caenorhabditis elegans by modulating DAF-16/FOXO. Biogerontology 20, 665-676. | no | ||||||||||||||||||||
1 mg/ml | whole life; start: n.d. | OP50 | NGM agar | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | up | yes | A3, pre-exposed to heat stress | Zeng L, Sun C, Pei Z, Yun T, Fan S, Long S, Wu T, Chen Z, Yang Z, Xu F (2019) Liangyi Gao extends lifespan and exerts an antiaging effect in Caenorhabditis elegans by modulating DAF-16/FOXO. Biogerontology 20, 665-676. | no | ||||||||||||||||||||||||||||||||||||||||||||||
1 mg/ml | whole life; start: n.d. | OP50 | NGM agar | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | n.d. | A3 | Zeng L, Sun C, Pei Z, Yun T, Fan S, Long S, Wu T, Chen Z, Yang Z, Xu F (2019) Liangyi Gao extends lifespan and exerts an antiaging effect in Caenorhabditis elegans by modulating DAF-16/FOXO. Biogerontology 20, 665-676. | no |