Treatment | Menadione | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CID (PubChem) | 4055 | ChEMBL | translocation of DAF-16 to nucleus | mean lifespan | maximum lifespan | |||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
100 µM | whole life; start: L4/A1 | OP50; from A12: OP50-1 | NGM agar | streptomycin (from A12) | N2 (wild type) | 20°C | down | -8.62 | yes | variable | -6.25 | n.d. | Urban N, Tsitsipatis D, Hausig F, Kreuzer K, Erler K, Stein V, Ristow M, Steinbrenner H, Klotz LO (2017) Non-linear impact of glutathione depletion on C. elegans life span and stress resistance. Redox Biol 11, 502-515. | |||||||||||
50 µM | 24 h; start: L1 | OP50 | NGM agar | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | none | no | L3 | Urban N, Tsitsipatis D, Hausig F, Kreuzer K, Erler K, Stein V, Ristow M, Steinbrenner H, Klotz LO (2017) Non-linear impact of glutathione depletion on C. elegans life span and stress resistance. Redox Biol 11, 502-515. | |||||||||||||||
100 µM | 24 h; start: L1 | OP50 | NGM agar | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | yes | L3 | Urban N, Tsitsipatis D, Hausig F, Kreuzer K, Erler K, Stein V, Ristow M, Steinbrenner H, Klotz LO (2017) Non-linear impact of glutathione depletion on C. elegans life span and stress resistance. Redox Biol 11, 502-515. | |||||||||||||||
200 µM | 24 h; start: L1 | OP50 | NGM agar | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | yes | L3 | Urban N, Tsitsipatis D, Hausig F, Kreuzer K, Erler K, Stein V, Ristow M, Steinbrenner H, Klotz LO (2017) Non-linear impact of glutathione depletion on C. elegans life span and stress resistance. Redox Biol 11, 502-515. |