Treatment | NG-094 | physical function | reproductive function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | locomotion on solid media | | | | number of offspring | | | | speed of larval development | | | | onset of egg laying | | | | quantity of polyQ aggregates | | | | activity / quantity / expression of HSPs | | | | activity / quantity / expression of HSPs | | | | activity / quantity / expression of HSPs | | | | mean lifespan | | | | maximum lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
0.25 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM134 {rmIs126 [unc-54p::Q0::YFP]} | 22°C | none | | n.d. | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
0.5 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM134 {rmIs126 [unc-54p::Q0::YFP]} | 22°C | none | | n.d. | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
0.75 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM134 {rmIs126 [unc-54p::Q0::YFP]} | 22°C | down | | n.d. | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
1.0 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM134 {rmIs126 [unc-54p::Q0::YFP]} | 22°C | up | | yes | A3; decreased motility (not significant) at A1 | | | | | | | | | | | | | | | | | up | | n.d. | A1; western blot analysis; up-regulation after 29°C heat shock for 3h; unchanged without heat shock; RT-PCR didn't show any changes for hsp-16.2 as well as for hsp-40, hsp-70, hsp-16.11, and hsp-90 | none | | n.d. | A1; western blot analysis; unchanged regulation after 29°C heat shock for 3h as well as without heat shock; RT-PCR didn't show any changes for hsp-40 as well as for hsp-16.2, hsp-70, hsp-16.11, and hsp-90 | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
1.25 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM134 {rmIs126 [unc-54p::Q0::YFP]} | 22°C | down | | n.d. | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
0.25 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 22°C | none | | n.d. | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
0.5 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 22°C | up | | n.d. | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
0.75 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 22°C | up | | n.d. | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
1.0 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 22°C | up | | yes | A1; also significantly increased at A3 | | | | | | | | | | | | | down | -20.00 | yes | | variable | | n.d. | A1; western blot analysis; up-regulation after 29°C heat shock for 3h; down-regulation without heat shock; RT-PCR didn't show any changes for hsp-16.2 as well as for hsp-40, hsp-70, hsp-16.11, and hsp-90 | down | | n.d. | A1; western blot analysis; slightly down-regulation after 29°C heat shock for 3h as well as without heat shock; RT-PCR didn't show changes for hsp-40 as well as for hsp-16.2, hsp-70, hsp-16.11, and hsp-90 | | | | | | | | | | | | | | | | HSF-1 | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
1.25 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 22°C | up | | n.d. | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
1.0 mM | whole life; start: unknown | OP50 | NGM agar | | AM134 {rmIs126 [unc-54p::Q0::YFP]} | 22°C | | | | | down | -10.00 | yes | | down | | n.d. | | delayed | | n.d. | | | | | | | | | | | | | | | | | | up | 3.47 | no | | none | 0 | no | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
1.0 mM | whole life; start: unknown | OP50 | NGM agar | | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 22°C | | | | | down | -20.00 | yes | | down | | n.d. | | delayed | | n.d. | | | | | | | | | | | | | | | | | | up | 7.04 | no | | up | 17.61 | yes | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
1.0 mM | whole life; start: unknown | OP50 | NGM agar | FUDR | BC10060 {dpy-5(e907); sEx884 [rCesC12C8.1::GFP + pCeh361]} | 22°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | A1; western blot analysis; after 29°C heat shock for 3h | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
1.0 mM | whole life; start: A1 | HT115 (DE3) with RNAi-vector against hsf-1 | NGM agar | FUDR | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 20°C | none | | no | A3 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |
1.0 mM | whole life; start: unknown | HT115 (DE3) with RNAi-vector against daf-16 | NGM agar | FUDR | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 22°C | up | | yes | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Haldimann P, Muriset M, Vígh L, Goloubinoff P. Haldimann P, Muriset M, Vígh L, Goloubinoff P (2011) The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans. J Biol Chem 286, 18784-18794. | |