Compound ID 252 (prev - next)

Name: NT219 (edit)

18 entries:

Treatment	NT219						physical function				physiological function								healthspan biomarkers																				lifespan				Transcriptome	Target	Reference
CID (PubChem)		ChEMBL					locomotion on solid media				heat stress resistance				UV stress resistance				quantity of β-Amyloid				activity / quantity / expression of HSPs				activity / quantity / expression of HSPs				quantity of polyQ aggregates				translocation of DAF-16 to nucleus				mean lifespan
Concentration	Exposure time	Food	Growth medium	Special additives	C. elegans strain	Temperature	Change compared to control	Percentual change compared to control	p < 0.05	notes	Change compared to control	Percentual change compared to control	p < 0.05	notes	Change compared to control	Percentual change compared to control	p < 0.05	notes	Change compared to control	Percentual change compared to control	p < 0.05	notes	Change compared to control	Percentual change compared to control	p < 0.05	notes	Change compared to control	Percentual change compared to control	p < 0.05	notes	Change compared to control	Percentual change compared to control	p < 0.05	notes	Change compared to control	Percentual change compared to control	p < 0.05	notes	Change compared to control	Percentual change compared to control	p < 0.05	notes
600 µM	3 h daily from A1-A6	HT115 (DE3)	NGM agar	ampicillin, IPTG	N2 (wild type)	20°C																																	none		no				El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
150 µM	3 h at A1 and 3 h at A2	HT115 (DE3)	NGM agar	ampicillin, IPTG	CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]}	20°C																	none		no	A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot																			El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
300 µM	3 h at A1 and 3 h at A2	HT115 (DE3)	NGM agar	ampicillin, IPTG	CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]}	20°C																	up		no	A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot																			El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
600 µM	3 h at A1 and 3 h at A2	HT115 (DE3)	NGM agar	ampicillin, IPTG	CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]}	20°C																	up		yes	A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot																			El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
900 µM	3 h at A1 and 3 h at A2	HT115 (DE3)	NGM agar	ampicillin, IPTG	CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]}	20°C																	none		no	A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot																			El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
150 µM	3 h at A1 and 3 h at A2	HT115 (DE3)	NGM agar	ampicillin, IPTG	BC10061 {sEx888 [hsp-70p (c12c8.1)::GFP]}	20°C																					none		n.d.	A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot															El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
300 µM	3 h at A1 and 3 h at A2	HT115 (DE3)	NGM agar	ampicillin, IPTG	BC10061 {sEx888 [hsp-70p (c12c8.1)::GFP]}	20°C																					none		n.d.	A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot															El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
600 µM	3 h at A1 and 3 h at A2	HT115 (DE3)	NGM agar	ampicillin, IPTG	BC10061 {sEx888 [hsp-70p (c12c8.1)::GFP]}	20°C																					up		n.d.	A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot															El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
600 µM	3 h at A1 and 3 h at A2	HT115 (DE3)	NGM agar	ampicillin, IPTG	CF512 [fer-15(b26); fem-1(hc17)]	20°C					up	32.04	yes	adults; stressor: 35°C for 15h; endpoint: survival	up	24.61	yes	A1; stressor: UV-light; endpoint: survival																					none		no		up-regulation of sod-3, hsp-12.6, hsp-70, hsp-16.1, gst-10, and gst-4	insulin/IGF signaling	El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
600 µM	3 h daily from A1-A6	HT115 (DE3)	NGM agar	ampicillin, IPTG	CF512 [fer-15(b26); fem-1(hc17)]	20°C																																	none		no				El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
600 µM	3 h; start unknown	HT115 (DE3)	NGM agar	ampicillin, IPTG	TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]}	20°C																													up		n.d.								El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
600 µM	3 h daily; start: A1	HT115 (DE3)	NGM agar	ampicillin, IPTG	CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]}	20°C	up	51.06	yes	A12; ratio of non-paralized and paralyzed nematodes; increased velocity also in A6-A11; non-significantly increased in A3-A5																																			El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
600 µM	3 h daily; start: A1	HT115 (DE3) with RNAi-vector against daf-16	NGM agar	ampicillin, IPTG	CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]}	20°C	none		n.d.	A3-A12; ratio of non-paralized and paralyzed nematodes									up		n.d.																								El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
600 µM	3 h daily; start: A1	HT115 (DE3)	NGM agar	ampicillin, IPTG	unknown strain; genotype: [polyQ40::YFP]	20°C																									down	-17.09	yes	adults; unchanged aggregation rate of polyQ40::YFP in A2											El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.
600 µM	3 h daily; start: A1	HT115 (DE3)	NGM agar	ampicillin, IPTG	unknown strain {polyQ35::YFP}	20°C	up	273.86	yes	A11																																			El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174.