| Treatment | Orange extract | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CID (PubChem) | ChEMBL | locomotion on solid media | body length / growth rate | number of offspring | heat stress resistance | UV stress resistance | ROS level | activity / quantity / expression of SODs | activity / quantity / expression of CTLs | autofluorescence (age pigment / lipofuscin) | MDA level | mean lifespan | maximum lifespan | |||||||||||||||||||||||||||||||||||||||||||||||
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | ||||||
| 100 mg/ml | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | up | n.d. | A9, A14, A18 | none | no | A5 | up | 9.8 | yes | A5; stressor: 35°C; endpoint: mean survival | up | 9.1 | no | A5; stressor: UV-B; endpoint: mean survival | down | -20.5 | yes | A5; measured via H2DCF-DA | up | 10 | no | A5 | up | 90 | yes | A5 | down | -18.3 | no | A5; blue fluorescence | down | -20.7 | yes | A5 | up | 10.5 | yes | up | 9.1 | n.d. | downregulation of age-1 | Wang J, Deng N, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans. Molecules 25, 351. | no | |||||||||||
| 200 mg/ml | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | up | n.d. | A9, A14, A18 | down | no | A5 | up | 19.3 | yes | A5; stressor: 35°C; endpoint: mean survival | up | 22 | yes | A5; stressor: UV-B; endpoint: mean survival | down | -28.4 | yes | A5; measured via H2DCF-DA | up | 70 | yes | A5 | up | 140 | yes | A5 | down | -22.5 | no | A5; blue fluorescence | down | yes | A5 | up | 18 | yes | up | 16.9 | n.d. | downregulation of age-1; upregulation of sod-3, skn-1, sek-1 | Wang J, Deng N, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans. Molecules 25, 351. | no | ||||||||||||
| 400 mg/ml | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | up | n.d. | A9, A14, A18 | down | no | A5 | up | 35.5 | yes | A5; stressor: 35°C; endpoint: mean survival | up | 36.1 | yes | A5; stressor: UV-B; endpoint: mean survival | down | -43.3 | yes | A5; measured via H2DCF-DA | up | 190 | yes | A5 | up | 220 | yes | A5 | down | -34.4 | yes | A5; blue fluorescence | down | -61.1 | yes | A5 | up | 26.2 | yes | up | 25.9 | n.d. | downregulation of age-1; upregulation of sod-3, daf-16, gst-4, skn-1, sek-1 | Wang J, Deng N, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans. Molecules 25, 351. | no | |||||||||||
| 100 mg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | Wang J, Deng N, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans. Molecules 25, 351. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||
| 200 mg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | Wang J, Deng N, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans. Molecules 25, 351. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||
| 400 mg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | Wang J, Deng N, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans. Molecules 25, 351. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||