Treatment | Pinoresinol | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CID (PubChem) | 73399 | ChEMBL | heat stress resistance | ROS level | translocation of DAF-16 to nucleus | median lifespan | mean lifespan | |||||||||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
100 µM | whole life; start: L4/A1 | OP50-1 | liquid NGM | streptomycin, BSA | N2 (wild type) | 20°C | none | no | A3; stressor: 37°C; endpoint: mean and median lifespan | none | no | A3; pre-exposed to 37°C; measured via H2DCF-DA | insulin-like signalling pathway | Koch K, Büchter C, Havermann S, Wätjen W (2015) The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. Phytother Res 29, 894-901. | ||||||||||||||||||
100 µM | whole life; start: L4/A1 | OP50-1 | liquid NGM | streptomycin, BSA; FUDR | N2 (wild type) | 25°C | up | 4.55 | n.d. | up | 4.55 | no | Koch K, Büchter C, Havermann S, Wätjen W (2015) The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. Phytother Res 29, 894-901. | |||||||||||||||||||
100 µM | whole life; start: L4/A1 | OP50-1 | liquid NGM | streptomycin, BSA; FUDR | TK22 [mev-1(kn1)] | 25°C | none | n.d. | down | -7.14 | no | Koch K, Büchter C, Havermann S, Wätjen W (2015) The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. Phytother Res 29, 894-901. | ||||||||||||||||||||
100 µM | whole life; start: L4/A1 | OP50-1 | liquid NGM | streptomycin, BSA | TK22 [mev-1(kn1)] | 20°C | up | 15.60 | yes | A3; stressor: 37°C; endpoint: mean and median lifespan | down | -13.30 | no | A3; pre-exposed to 37°C; measured via H2DCF-DA | Koch K, Büchter C, Havermann S, Wätjen W (2015) The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. Phytother Res 29, 894-901. | |||||||||||||||||
25 µM | 1 h; start: L4/A1 | OP50-1 | liquid NGM | streptomycin, BSA | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | no | Koch K, Büchter C, Havermann S, Wätjen W (2015) The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. Phytother Res 29, 894-901. | |||||||||||||||||||||||
50 µM | 1 h; start: L4/A1 | OP50-1 | liquid NGM | streptomycin, BSA | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | yes | Koch K, Büchter C, Havermann S, Wätjen W (2015) The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. Phytother Res 29, 894-901. | |||||||||||||||||||||||
100 µM | 1 h; start: L4/A1 | OP50-1 | liquid NGM | streptomycin, BSA | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | yes | Koch K, Büchter C, Havermann S, Wätjen W (2015) The Lignan Pinoresinol Induces Nuclear Translocation of DAF-16 in Caenorhabditis elegans but has No Effect on Life Span. Phytother Res 29, 894-901. |