Treatment | Platycladus orientalis extract (n-butanol extract from seed) | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||
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CID (PubChem) | ChEMBL | heat stress resistance | oxidative stress resistance | autofluorescence (age pigment / lipofuscin) | ROS level | activity / quantity / expression of HSPs | activity / quantity / expression of GSTs | mean lifespan | ||||||||||||||||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
20 µg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | up | 1.67 | no | Liu H, Liang F, Su W, Wang N, Lv M, Li P, Pei Z, Zhang Y, Xie XQ, Wang L, Wang Y (2013) Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans. J Ethnopharmacol 147, 366-372. | yes | ||||||||||||||||||||||||||||||
100 µg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | up | 9.91 | yes | Liu H, Liang F, Su W, Wang N, Lv M, Li P, Pei Z, Zhang Y, Xie XQ, Wang L, Wang Y (2013) Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans. J Ethnopharmacol 147, 366-372. | yes | ||||||||||||||||||||||||||||||
500 µg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | up | 17.70 | yes | A3; Stressor: 35°C for 1 h; endpoint: mean lifespan | up | 62.00 | yes | A2; Stressor: 500 µM juglone for 5 h | down | -41.7 | yes | A10 | down | -34.3 | yes | A2; measured with DCF-DA | up | 24.54 | yes | Liu H, Liang F, Su W, Wang N, Lv M, Li P, Pei Z, Zhang Y, Xie XQ, Wang L, Wang Y (2013) Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans. J Ethnopharmacol 147, 366-372. | yes | ||||||||||||||
500 µg/ml | whole life; start: L1 | OP50 | NGM agar | CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]} | 20°C | up | 122 | yes | A4; 1 day before measurement: heat shock at 25°C for 30 min and 35°C for 1 h) | Liu H, Liang F, Su W, Wang N, Lv M, Li P, Pei Z, Zhang Y, Xie XQ, Wang L, Wang Y (2013) Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans. J Ethnopharmacol 147, 366-372. | yes | |||||||||||||||||||||||||||||
500 µg/ml | whole life; start: L1 | OP50 | NGM agar | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | up | 129.00 | yes | A1; measured via fluorescence | Liu H, Liang F, Su W, Wang N, Lv M, Li P, Pei Z, Zhang Y, Xie XQ, Wang L, Wang Y (2013) Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans. J Ethnopharmacol 147, 366-372. | yes |