Treatment | Quercetin | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 5280343 | ChEMBL | CHEMBL50 | | | | locomotion on solid media | | | | heat stress resistance | | | | oxidative stress resistance | | | | autofluorescence (age pigment / lipofuscin) | | | | ROS level | | | | quantity of β-Amyloid | | | | activity / quantity / expression of SODs | | | | activity / quantity / expression of GSTs | | | | translocation of DAF-16 to nucleus | | | | median lifespan | | | | mean lifespan | | | | maximum lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
50 µg/ml | whole life; start: A1 | OP50 | NGM agar | | N2 (wild type) | 22 °C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -8.33 | no | significance was determined for mean lifespan (log rank test) | | | | | down | -16.67 | | | | | | | Wu Z, Smith JV, Paramasivam V, Butko P, Khan I, Cypser JR, Luo Y (2002) Ginkgo biloba extract EGb 761 increases stress resistance and extends life span of Caenorhabditis elegans. Cell Mol Biol (Noisy-le-grand) 48, 725-731. | |
100 µM | whole life; start: L1 | OP50-1 | liquid NGM | Streptomycin | N2 (wild type) | 20°C | | | | | | | | | up | 19.62 | yes | A1; Stressor: 150 µM Juglone | | | | | | | | | | | | | | | | | | | | | | | | | up | 19.05 | yes | | up | 15.17 | yes | | up | 14.97 | n.d. | | | | | DAF-16 | Kampkötter A, Timpel C, Zurawski RF, Ruhl S, Chovolou Y, Proksch P, Wätjen W (2008) Increase of stress resistance and lifespan of Caenorhabditis elegans by quercetin. Comp Biochem Physiol B Biochem Mol Biol 149, 314-323. | |
100 µM | whole life; start: L1 | OP50-1 | liquid NGM | Streptomycin | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | down | -15.00 | yes | A1; SOD-3-promoter activity measured by fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | Kampkötter A, Timpel C, Zurawski RF, Ruhl S, Chovolou Y, Proksch P, Wätjen W (2008) Increase of stress resistance and lifespan of Caenorhabditis elegans by quercetin. Comp Biochem Physiol B Biochem Mol Biol 149, 314-323. | |
100 µM | whole life; start: L1 | OP50-1 | liquid NGM | Streptomycin | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A1 | | | | | | | | | | | | | | | | | Kampkötter A, Timpel C, Zurawski RF, Ruhl S, Chovolou Y, Proksch P, Wätjen W (2008) Increase of stress resistance and lifespan of Caenorhabditis elegans by quercetin. Comp Biochem Physiol B Biochem Mol Biol 149, 314-323. | |
50 µg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | up | 14.2 | yes | A5; Stressor: 35°C; endpoint: mean lifespan; median lifespan is also significantly increased | up | 65.8 | yes | A2; Stressor: 0.5 mM H2O2 for 8 h | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |
50 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | N2 (wild type) | 20°C | | | | | up | 8.30 | no | A2; stressor: 37°C; endpoint: mean lifespan; similar change in median lifespan | | | | | | | | | down | | yes | A2; exposed to 37°C during measurement; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | N2 (wild type) | 20°C | | | | | up | 14.11 | yes | A2; stressor: 37°C; endpoint: mean lifespan; similar change in median lifespan | | | | | down | -27.85 | yes | A7; blue fluorescence | down | | yes | A2; exposed to 37°C during measurement; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A5; sod-3 expression; pre-exposed to juglone; unchanged without juglone exposure; measured via fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A5; gst-4 expression; pre-exposed to juglone; no significant change without pre-exposure; measured via fluorescence | | | | | | | | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 27.78 | n.d. | | up | 18.92 | yes | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | CF1038 [daf-16(mu86)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 27.27 | n.d. | | up | 14.39 | yes | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
200 µg/mL | whole life; start: egg stage | OP50 | NGM agar | Gentamycin | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 20°C | up | | no | L3; temperature shift to 23°C prior analysing beta-amyloid induced paralysis | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sangha, J. S., Sun, X., Wally, O. S., Zhang, K., Ji, X., Wang, Z., ... & Zhang, J. (2012). Liuwei Dihuang (LWDH), a traditional Chinese medicinal formula, protects against β-amyloid toxicity in transgenic Caenorhabditis elegans. PLoS One, 7(8). | |
200 µg/mL | whole life; start: egg stage | OP50 | NGM agar | Gentamycin | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | | | | | | | | | | | | | | | | | | | | | down | | no | A4 & A8 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sangha, J. S., Sun, X., Wally, O. S., Zhang, K., Ji, X., Wang, Z., ... & Zhang, J. (2012). Liuwei Dihuang (LWDH), a traditional Chinese medicinal formula, protects against β-amyloid toxicity in transgenic Caenorhabditis elegans. PLoS One, 7(8). | |
200 µg/mL | whole life; start: egg stage | OP50 | NGM agar | Gentamycin | N2 (wild type) | 20°C | | | | | | | | | up | | yes | L4; stressor: 300 µM juglone; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sangha, J. S., Sun, X., Wally, O. S., Zhang, K., Ji, X., Wang, Z., ... & Zhang, J. (2012). Liuwei Dihuang (LWDH), a traditional Chinese medicinal formula, protects against β-amyloid toxicity in transgenic Caenorhabditis elegans. PLoS One, 7(8). | |
100 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | up | 8,8 | yes | A1; stressor: 37°C; endpoint: survival | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Zhou, J. B., Zheng, Y. L., Zeng, Y. X., Wang, J. W., Pei, Z., & Pang, J. Y. (2018). Marine derived xyloketal derivatives exhibit anti-stress and anti-ageing effects through HSF pathway in Caenorhabditis elegans. European journal of medicinal chemistry, 148, 63-72. | |