Treatment | Red mold dioscorea (ethanol extract) | physiological function | healthspan biomarkers | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | |||||||||||||||||||||||||||
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CID (PubChem) | ChEMBL | oxidative stress resistance | oxidative stress resistance | oxidative stress resistance | ROS level | activity / quantity / expression of SODs | translocation of DAF-16 to nucleus | |||||||||||||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | N2 (wild type) | 20°C | up | yes | A1; stressor: 250 µM juglone for 3.5 h; no change by the use of other juglone concentrations; endpoint: survival | down | yes | A1; measured via H2DCF-DA | Shi YC, Yu CW, Liao VH, Pan TM (2012) Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PLoS One 7, e39515. | ||||||||||||||||||||||||
10 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | N2 (wild type) | 20°C | up | yes | A1; stressor: 250 µM juglone for 3.5 h; no change by the use of other juglone concentrations; endpoint: survival | up | yes | A1; stressor: 1 mM H2O2 for 4.5 h; endpoint: survival | up | yes | A1; stressor: 150 mM paraquat for 12 h; endpoint: survival | down | yes | A1; measured via H2DCF-DA | up-regulation of sod-3 | DAF-16, SOD-3 | Shi YC, Yu CW, Liao VH, Pan TM (2012) Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PLoS One 7, e39515. | ||||||||||||||||
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | N2 (wild type) | 20°C | up | yes | A1; stressor: 250 µM juglone for 3.5 h; no change by the use of other juglone concentrations; endpoint: survival | down | yes | A1; measured via H2DCF-DA | Shi YC, Yu CW, Liao VH, Pan TM (2012) Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PLoS One 7, e39515. | ||||||||||||||||||||||||
10 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | GR1307 [daf-16(mgDf50)] | 20°C | none | no | A1; stressor: 250 µM juglone for 3.5 h; endpoint: survival | Shi YC, Yu CW, Liao VH, Pan TM (2012) Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PLoS One 7, e39515. | |||||||||||||||||||||||||||
10 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | CF1038 [daf-16(mu86)] | 20°C | none | no | A1; stressor: 250 µM juglone for 3.5 h; endpoint: survival | Shi YC, Yu CW, Liao VH, Pan TM (2012) Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PLoS One 7, e39515. | |||||||||||||||||||||||||||
10 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | TK22 [mev-1(kn1)] | 20°C | up | yes | A1; stressor: 250 µM juglone for 3.5 h; endpoint: survival | Shi YC, Yu CW, Liao VH, Pan TM (2012) Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PLoS One 7, e39515. | |||||||||||||||||||||||||||
10 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | up | yes | A1; SOD-3 expression; similar change when pre-exposed to juglone; measured via fluorescence | Shi YC, Yu CW, Liao VH, Pan TM (2012) Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PLoS One 7, e39515. | |||||||||||||||||||||||||||
10 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | yes | A1; similar result with and without pre-exposure to juglone | Shi YC, Yu CW, Liao VH, Pan TM (2012) Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PLoS One 7, e39515. |