Treatment | Resveratrol | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 445154 | ChEMBL | CHEMBL165 | | | | pharyngeal pumping | | | | locomotion in liquid media | | | | body length / growth rate | | | | number of offspring | | | | quantity of late reproduction | | | | quantity of early reproduction | | | | onset of egg laying | | | | heat stress resistance | | | | oxidative stress resistance | | | | autofluorescence (age pigment / lipofuscin) | | | | ROS level | | | | activity / quantity / expression of HSPs | | | | activity / quantity / expression of SODs | | | | activity / quantity / expression of GSTs | | | | level of autophagy | | | | translocation of SKN-1 to nucleus | | | | translocation of DAF-16 to nucleus | | | | median lifespan | | | | mean lifespan | | | | maximum lifespan | | | | minimum lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
50 µM | whole life; start: egg stage | OP50, heat-inactivated | NGM agar | | N2 (wild type) | 18°C | none | | no | A1 - A10 | | | | | none | | no | A2 & A4 | down | | no | | up | | yes | A5 - A9 | down | -20.00 | yes | A0 - A4 | earlier | -15.00 | yes | | none | | no | Stressor: 35°C; one experiment showed detrimental effects of resveratrol on heat resistance | up | 24.59 | yes | A2; Stressor: 10 mM Paraquat | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 50.85 | yes | | up | 29.24 | yes | | up | 96.98 | n.d. | Start of dying phase | | | | | Gruber J, Tang SY, Halliwell B (2007) Evidence for a trade-off between survival and fitness caused by resveratrol treatment of Caenorhabditis elegans. Ann N Y Acad Sci 1100, 530-542. | |
100 µg/ml | whole life; start: unknown | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | Measured with a DsRed::LGG-1 transgene reporter gene product | | | | | | | | | | | | | up | 10.96 | n.d. | | up | 11.54 | n.d. | | | | | | | | | sir-2.1 and the autophagy pathway | Morselli E, Maiuri MC, Markaki M, Megalou E, Pasparaki A, Palikaras K, Criollo A, Galluzzi L, Malik SA, Vitale I, Michaud M, Madeo F, Tavernarakis N, Kroemer G (2010) Caloric restriction and resveratrol promote longevity through the Sirtuin-1-dependent induction of autophagy. Cell Death Dis 1:e10, | |
100 µg/ml | whole life; start: unknown | OP50 | NGM agar | | VC199 [sir-2.1(ok434)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | Measured with a DsRed::LGG-1 transgene reporter gene product | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Morselli E, Maiuri MC, Markaki M, Megalou E, Pasparaki A, Palikaras K, Criollo A, Galluzzi L, Malik SA, Vitale I, Michaud M, Madeo F, Tavernarakis N, Kroemer G (2010) Caloric restriction and resveratrol promote longevity through the Sirtuin-1-dependent induction of autophagy. Cell Death Dis 1:e10, | |
100 µg/ml | whole life; start: unknown | HT115 (DE3) with RNAi-vector against bec-1 | NGM agar | IPTG | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -1.53 | n.d | | up | 09.09 | n.d. | | | | | | | | | | Morselli E, Maiuri MC, Markaki M, Megalou E, Pasparaki A, Palikaras K, Criollo A, Galluzzi L, Malik SA, Vitale I, Michaud M, Madeo F, Tavernarakis N, Kroemer G (2010) Caloric restriction and resveratrol promote longevity through the Sirtuin-1-dependent induction of autophagy. Cell Death Dis 1:e10, | |
18 µM | whole life; start: A1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -12.50 | yes | A2; Stressor: 35°C; stress exposure in S-medium; endpoint: median lifespan; 1 out of 2 trials were significant; percentual change = average of 2 trials | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Benedetti MG, Foster AL, Vantipalli MC, White MP, Sampayo JN, Gill MS, Olsen A, Lithgow GJ (2008) Compounds that confer thermal stress resistance and extended lifespan. Exp Gerontol 43, 882-891. | |
438 µM | whole life; start: A1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -10.00 | yes | A2; Stressor: 35°C; stress exposure in S-medium; endpoint: median lifespan; 1 out of 2 trials were significant; percentual change = average of 2 trials | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Benedetti MG, Foster AL, Vantipalli MC, White MP, Sampayo JN, Gill MS, Olsen A, Lithgow GJ (2008) Compounds that confer thermal stress resistance and extended lifespan. Exp Gerontol 43, 882-891. | |
100 µM | | OP50-1 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 14.2 | yes | percentual change in a second trial: 14.6 | | | | | | | | | | | | aak-2 / AMPK | Greer EL, Brunet A (2009) Different dietary restriction regimens extend lifespan by both independent and overlapping genetic pathways in C. elegans. Aging Cell 8, 113-127. | |
100 µM | | OP50-1 | NGM agar | | RB754 [aak-2(ok524)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 2.2 | no | | | | | | | | | | | | | | Greer EL, Brunet A (2009) Different dietary restriction regimens extend lifespan by both independent and overlapping genetic pathways in C. elegans. Aging Cell 8, 113-127. | |
100 µM | | OP50-1 | NGM agar | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 13.7 | yes | | | | | | | | | | | | | | Greer EL, Brunet A (2009) Different dietary restriction regimens extend lifespan by both independent and overlapping genetic pathways in C. elegans. Aging Cell 8, 113-127. | |
50 µM | whole life; start: L1 | OP50 | NGM agar | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -25.2 | yes | A1; pre-incubation: 20 µM juglone for 24 h; measurement via fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
100 µM | whole life; start: L1 | OP50 | NGM agar | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -38.4 | yes | A1; pre-incubation: 20 µM juglone for 24 h; measurement via fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
200 µM | whole life; start: L1 | OP50 | NGM agar | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -40.7 | yes | A1; pre-incubation: 20 µM juglone for 24 h; measurement via fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
50 µM | whole life; start: A1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -0.4 | no | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
100 µM | whole life; start: A1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -4.0 | no | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
200 µM | whole life; start: A1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 0.5 | no | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
50 µM | whole life; start: A1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -3.9 | no | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
100 µM | whole life; start: A1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -18.6 | yes | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
200 µM | whole life; start: A1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -24.0 | yes | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
50 µM | whole life; start: A1 | OP50 | NGM agar | 50 mM glucose | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 10.9 | yes | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
100 µM | whole life; start: A1 | OP50 | NGM agar | 50 mM glucose | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 9.4 | no | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
200 µM | whole life; start: A1 | OP50 | NGM agar | 50 mM glucose | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 16.0 | yes | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
50 µM | whole life; start: A1 | OP50 | NGM agar | | TK22 [mev-1(kn1)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -3.5 | no | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
100 µM | whole life; start: A1 | OP50 | NGM agar | | TK22 [mev-1(kn1)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -6.2 | no | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
200 µM | whole life; start: A1 | OP50 | NGM agar | | TK22 [mev-1(kn1)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -9.6 | yes | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
50 µM | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 53.1 | yes | A1; Stressor: 400 µM juglone; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
100 µM | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 30.1 | yes | A1; Stressor: 400 µM juglone; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
200 µM | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 15.6 | yes | A1; Stressor: 400 µM juglone; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Chen W, Rezaizadehnajafi L, Wink M (2013) Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans. J Pharm Pharmacol 65, 682-688. | |
100 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 24.40 | yes | | | | | | | | | | | | | | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |
100 µM | whole life; start: egg stage | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 12.99 | yes | | up | | yes | | | | | | | | | | | | | | Chen Y, Onken B, Chen H, Xiao S, Liu X, Driscoll M, Cao Y, Huang Q (2014) Mechanism of longevity extension of Caenorhabditis elegans induced by pentagalloyl glucose isolated from eucalyptus leaves. J Agric Food Chem 62, 3422-3431. | |
100 µM | whole life; start: A1 | OP50, heat-inactivated | liquid NGM | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A3; stressor: 37°C; endpoint: mean lifespan | | | | | | | | | down | | no | A3; exposed to 37°C; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Fischer N, Büchter C, Koch K, Albert S, Csuk R, Wätjen W (2017) The resveratrol derivatives trans-3,5-dimethoxy-4-fluoro-4'-hydroxystilbene and trans-2,4',5-trihydroxystilbene decrease oxidative stress and prolong lifespan in Caenorhabditis elegans. J Pharm Pharmacol 69, 73-81. | |
100 µM | whole life; start: A1 | OP50, heat-inactivated | liquid NGM | FUDR | N2 (wild type) | 25°C | | | | | up | | no | A11; unchanged at A18 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | | | | | | | | | Fischer N, Büchter C, Koch K, Albert S, Csuk R, Wätjen W (2017) The resveratrol derivatives trans-3,5-dimethoxy-4-fluoro-4'-hydroxystilbene and trans-2,4',5-trihydroxystilbene decrease oxidative stress and prolong lifespan in Caenorhabditis elegans. J Pharm Pharmacol 69, 73-81. | |
100 µM | whole life; start: A1 | OP50, heat-inactivated | liquid NGM | FUDR | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A3; exposed to 37°C; measured via H2DCF-DA; significant change only during early time point | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Fischer N, Büchter C, Koch K, Albert S, Csuk R, Wätjen W (2017) The resveratrol derivatives trans-3,5-dimethoxy-4-fluoro-4'-hydroxystilbene and trans-2,4',5-trihydroxystilbene decrease oxidative stress and prolong lifespan in Caenorhabditis elegans. J Pharm Pharmacol 69, 73-81. | |
100 µM | whole life; start: A1 | OP50, heat-inactivated | liquid NGM | FUDR | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | no | A3; exposed to 37°C; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Fischer N, Büchter C, Koch K, Albert S, Csuk R, Wätjen W (2017) The resveratrol derivatives trans-3,5-dimethoxy-4-fluoro-4'-hydroxystilbene and trans-2,4',5-trihydroxystilbene decrease oxidative stress and prolong lifespan in Caenorhabditis elegans. J Pharm Pharmacol 69, 73-81. | |
100 µM | whole life; start: A1 | OP50, heat-inactivated | liquid NGM | FUDR | VC199 [sir-2.1(ok434)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A3; exposed to 37°C; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Fischer N, Büchter C, Koch K, Albert S, Csuk R, Wätjen W (2017) The resveratrol derivatives trans-3,5-dimethoxy-4-fluoro-4'-hydroxystilbene and trans-2,4',5-trihydroxystilbene decrease oxidative stress and prolong lifespan in Caenorhabditis elegans. J Pharm Pharmacol 69, 73-81. | |
100 µM | 1 h; start: A1 | OP50, heat-inactivated | liquid NGM | FUDR | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A1 | | | | | | | | | | | | | | | | | | | | | Fischer N, Büchter C, Koch K, Albert S, Csuk R, Wätjen W (2017) The resveratrol derivatives trans-3,5-dimethoxy-4-fluoro-4'-hydroxystilbene and trans-2,4',5-trihydroxystilbene decrease oxidative stress and prolong lifespan in Caenorhabditis elegans. J Pharm Pharmacol 69, 73-81. | |
100 µM | 1 h; start: A1 | OP50, heat-inactivated | liquid NGM | FUDR | LD001 {ldIs007 [skn-1p::SKN-1::gfp]; pRF4 [rol-6(su1006gf)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A1 | | | | | | | | | | | | | | | | | | | | | | | | | Fischer N, Büchter C, Koch K, Albert S, Csuk R, Wätjen W (2017) The resveratrol derivatives trans-3,5-dimethoxy-4-fluoro-4'-hydroxystilbene and trans-2,4',5-trihydroxystilbene decrease oxidative stress and prolong lifespan in Caenorhabditis elegans. J Pharm Pharmacol 69, 73-81. | |
50 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 7.88 | no | A2; stressor: 37°C; endpoint: mean lifespan; similar change in median lifespan | | | | | | | | | up | | no | A2; exposed to 37°C during measurement; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 9.75 | yes | A2; stressor: 37°C; endpoint: mean lifespan; similar change in median lifespan | | | | | down | -11.05 | yes | A7; blue fluorescence | up | | no | A2; exposed to 37°C during measurement; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A5; sod-3 expression; pre-exposed to juglone; unchanged without juglone exposure; measured via fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A5; gst-4 expression; unchanged when pre-exposed to juglone; significant change without pre-exposure; measured via fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 22.22 | n.d. | | up | 22.54 | yes | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | CF1038 [daf-16(mu86)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | n.d. | | up | 8.69 | yes | | | | | | | | | | | | | | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |