2 mg/ml | whole life; start: parental generation | OP50 | NGM agar | | N2 (wild type) | 20°C | down | -4.00 | no | | | | | | up | 1.00 | no | | | | | | Evason K, Huang C, Yamben I, Covey DF, Kornfeld K (2005) Anticonvulsant medications extend worm life-span. Science. 307, 258-262. | |
5 mM (UV-treated succinimide) | whole life; start: L4 | OP50, UV-inactivated | NGM agar | FUDR; ampicillin | N2 (wild type) | 20°C | | | | | none | | | A7 | | | | | | | | | Choi H, Schneider H, Klum S, Chandler-Brown D, Kaeberlein M, Shamieh L (2013) UV-photoconversion of ethosuximide from a longevity-promoting compound to a potent toxin. PLoS One 8, e82543. | |
10 mM (UV-treated succinimide) | whole life; start: L4 | OP50, UV-inactivated | NGM agar | FUDR; ampicillin | N2 (wild type) | 20°C | | | | | none | | | A7 | | | | | | | | | Choi H, Schneider H, Klum S, Chandler-Brown D, Kaeberlein M, Shamieh L (2013) UV-photoconversion of ethosuximide from a longevity-promoting compound to a potent toxin. PLoS One 8, e82543. | |
50 mM (UV-treated succinimide) | whole life; start: L4 | OP50, UV-inactivated | NGM agar | FUDR; ampicillin | N2 (wild type) | 20°C | | | | | none | | | A7 | | | | | | | | | Choi H, Schneider H, Klum S, Chandler-Brown D, Kaeberlein M, Shamieh L (2013) UV-photoconversion of ethosuximide from a longevity-promoting compound to a potent toxin. PLoS One 8, e82543. | |
5 mM | whole life; start: L4 | OP50, UV-inactivated | NGM agar | FUDR; ampicillin | N2 (wild type) | 20°C | | | | | none | | | A7 | | | | | | | | | Choi H, Schneider H, Klum S, Chandler-Brown D, Kaeberlein M, Shamieh L (2013) UV-photoconversion of ethosuximide from a longevity-promoting compound to a potent toxin. PLoS One 8, e82543. | |
10 mM | whole life; start: L4 | OP50, UV-inactivated | NGM agar | FUDR; ampicillin | N2 (wild type) | 20°C | | | | | none | | | A7 | | | | | | | | | Choi H, Schneider H, Klum S, Chandler-Brown D, Kaeberlein M, Shamieh L (2013) UV-photoconversion of ethosuximide from a longevity-promoting compound to a potent toxin. PLoS One 8, e82543. | |
50 mM | whole life; start: L4 | OP50, UV-inactivated | NGM agar | FUDR; ampicillin | N2 (wild type) | 20°C | | | | | none | | | A7 | | | | | | | | | Choi H, Schneider H, Klum S, Chandler-Brown D, Kaeberlein M, Shamieh L (2013) UV-photoconversion of ethosuximide from a longevity-promoting compound to a potent toxin. PLoS One 8, e82543. | |