Treatment | TSG (2,3,5,4-Tetrahydroxystilbene-2-O-𝛽-D-glucoside) from Polygonum multiflorum | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||
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CID (PubChem) | 5321884 | ChEMBL | heat stress resistance | autofluorescence (age pigment / lipofuscin) | ROS level | activity / quantity / expression of SODs | activity / quantity / expression of GSTs | median lifespan | mean lifespan | |||||||||||||||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
50 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | N2 (wild type) | 20°C | up | 22.20 | yes | A2; stressor: 37°C; endpoint: mean lifespan; similar change in median lifespan | none | no | A2; exposed to 37°C during measurement; measured via H2DCF-DA | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no | |||||||||||||||||||||||||
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | N2 (wild type) | 20°C | up | 23.24 | yes | A2; stressor: 37°C; endpoint: mean lifespan; similar change in median lifespan | down | -20.00 | yes | A7; blue fluorescence | down | -10.40 | variable | A2; exposed to 37°C during measurement; measured via H2DCF-DA; significant changes only after 60 minutes | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no | ||||||||||||||||||||
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | N2 (wild type) | 25°C | up | 22.22 | n.d. | up | 21.56 | yes | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no | ||||||||||||||||||||||||||
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | CF1038 [daf-16(mu86)] | 25°C | up | 9.09 | n.d. | up | 10.50 | yes | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no | ||||||||||||||||||||||||||
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | down | -23.91 | yes | A5; sod-3 expression; pre-exposed to juglone; unchanged without juglone exposure; measured via fluorescence | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no | ||||||||||||||||||||||||||||
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | down | yes | A5; gst-4 expression; unchanged when pre-exposed to juglone; significant change without pre-exposure; measured via fluorescence | Büchter C, Zhao L, Havermann S, Honnen S, Fritz G, Proksch P, Wätjen W (2015) TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans. Oxid Med Cell Longev 2015:124357. | no |