Treatment | Tyrosol | cognitive function | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 10393 | ChEMBL | | | | | neuronal survival / integrity | | | | pharyngeal pumping | | | | locomotion in liquid media | | | | body length / growth rate | | | | number of offspring | | | | vertex (progeny per day) | | | | speed of larval development | | | | onset of egg laying | | | | heat stress resistance | | | | oxidative stress resistance | | | | ROS level | | | | quantity of α-synuclein | | | | translocation of DAF-16 to nucleus | | | | median lifespan | | | | mean lifespan | | | | maximum lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
100 µM | whole life; start: egg stage | OP50 | NGM agar | | DH26 [fer-15(b26)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 6.86 | no | | | | | | | | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
250 µM | whole life; start: egg stage | OP50 | NGM agar | | DH26 [fer-15(b26)] | 25°C | | | | | up | | yes | A2 - A9; no significant change at A1 | | | | | down | | yes | L4; no significant changes at A1 and A5 | | | | | | | | | | | | | | | | | up | 55.43 | yes | A5; stressor: 35°C for 8 h; endpoint: survival | up | | yes | A1; stressor: 4 mM paraquat; endpoint: mean lifespan | | | | | | | | | | | | | up | 20.63 | yes | | up | 21.13 | yes | | up | 10.76 | yes | | A4: up-regulation of hsp-12.6 | A4: up-regulation of GEI-7, EF-2, SAHH, ENPL-1, VHA-12, VHA-13, GST-4; down-regulation of VIT-2, RAN-1, ACDH-3, ADH-1. Isoform distribution changed in VIT-5 and HSP-4 | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
1 mM | whole life; start: egg stage | OP50 | NGM agar | | DH26 [fer-15(b26)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 5.44 | no | | | | | | | | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
2.5 mM | whole life; start: egg stage | OP50 | NGM agar | | DH26 [fer-15(b26)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 4.85 | no | | | | | | | | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
250 µM | whole life; start: egg stage | OP50 | NGM agar | | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | none | | no | | none | | no | | none | | n.d. | | none | | n.d. | | up | | yes | A5; stressor: 35°C for 8 h; endpoint: survival | up | | yes | A1; stressor: 4 mM paraquat; endpoint: mean lifespan | | | | | | | | | | | | | up | 15.38 | yes | | up | 16.55 | yes | | | | | | up-regulation of hsp-12.6 | | | DAF-16 and HSF-1 | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
250 µM | whole life; start: L1 | OP50 | NGM agar | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | L4; intermediate nuclear translocation | | | | | | | | | | | | | | | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
250 µM | whole life; start: egg stage | OP50 | NGM agar | | TK22 [mev-1(kn1)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A1; stressor: 4 mM paraquat; endpoint: mean lifespan | | | | | | | | | | | | | up | 11.11 | no | | up | 4.69 | no | | | | | | | | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
250 µM | whole life; start: egg stage | OP50 | NGM agar | | VC199 [sir-2.1(ok434)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 9.09 | yes | | up | 9.36 | yes | | | | | | | | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
250 µM | whole life; start: egg stage | OP50 | NGM agar | | GM11 [daf-16(m26); fer-15(b26)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A5; stressor: 35°C for 8 h; endpoint: survival | none | | no | A1; stressor: 4 mM paraquat; endpoint: mean lifespan | | | | | | | | | | | | | up | 7.69 | no | | up | 1.46 | no | | | | | | | | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
250 µM | whole life; start: egg stage | OP50 | NGM agar | | GM6 [fer-15(b26); daf-2(e1370)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A5; stressor: 35°C for 8 h; endpoint: survival | none | | no | A1; stressor: 4 mM paraquat; endpoint: mean lifespan | | | | | | | | | | | | | up | 2.44 | no | | up | 6.13 | no | | | | | | | | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
250 µM | whole life; start: egg stage | OP50 | NGM agar | | PS3551 [hsf-1(sy441)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A5; stressor: 35°C for 8 h; endpoint: survival | none | | no | A1; stressor: 4 mM paraquat; endpoint: mean lifespan | | | | | | | | | | | | | none | | no | | up | 7.43 | no | | | | | | | | | | Cañuelo A, Gilbert-López B, Pacheco-Liñán P, Martínez-Lara E, Siles E, Miranda-Vizuete A (2012)Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech Ageing Dev 133, 563-574. | |
50 µM | whole life; start: A1 | OP50 | liquid S-medium | FUdR, penicillin, streptomycin | NL5901 {pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | | | | | Garcia-Moreno JC, Porta de la Riva M, Martínez-Lara E, Siles E, Cañuelo A (2019) Tyrosol, a simple phenol from EVOO, targets multiple pathogenic mechanisms of neurodegeneration in a C. elegans model of Parkinson's disease. Neurobiol Aging 82, 60-68. | no |
250 µM | whole life; start: A1 | OP50 | liquid S-medium | FUdR, penicillin, streptomycin | NL5901 {pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | | | | | Garcia-Moreno JC, Porta de la Riva M, Martínez-Lara E, Siles E, Cañuelo A (2019) Tyrosol, a simple phenol from EVOO, targets multiple pathogenic mechanisms of neurodegeneration in a C. elegans model of Parkinson's disease. Neurobiol Aging 82, 60-68. | no |
500 µM | whole life; start: A1 | OP50 | liquid S-medium | FUdR, penicillin, streptomycin | NL5901 {pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | | | | | Garcia-Moreno JC, Porta de la Riva M, Martínez-Lara E, Siles E, Cañuelo A (2019) Tyrosol, a simple phenol from EVOO, targets multiple pathogenic mechanisms of neurodegeneration in a C. elegans model of Parkinson's disease. Neurobiol Aging 82, 60-68. | no |
1000 µM | whole life; start: A1 | OP50 | liquid S-medium | FUdR, penicillin, streptomycin | NL5901 {pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -34.1 | yes | A6; measured via H2DCF-DA | | | | | | | | | | | | | up | 14.2 | yes | | | | | | | | | | Garcia-Moreno JC, Porta de la Riva M, Martínez-Lara E, Siles E, Cañuelo A (2019) Tyrosol, a simple phenol from EVOO, targets multiple pathogenic mechanisms of neurodegeneration in a C. elegans model of Parkinson's disease. Neurobiol Aging 82, 60-68. | no |
1000 µM | whole life; start: L4 | OP50 | NGM agar | FUdR, penicillin, streptomycin | NL5901 {pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]} | 20°C | | | | | | | | | up | | yes | A9; no significance at A3, A7, A11 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -61.5 | yes | A6; via fluorescence; not confirmed via western blot | | | | | | | | | | | | | | | | | | | | | Garcia-Moreno JC, Porta de la Riva M, Martínez-Lara E, Siles E, Cañuelo A (2019) Tyrosol, a simple phenol from EVOO, targets multiple pathogenic mechanisms of neurodegeneration in a C. elegans model of Parkinson's disease. Neurobiol Aging 82, 60-68. | no |
1000 µM | whole life; start: A1 | OP50 | NGM agar | FUdR, penicillin, streptomycin | N2 (wild type) | 20°C | | | | | | | | | none | | no | A3, A7, A9, A11 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Garcia-Moreno JC, Porta de la Riva M, Martínez-Lara E, Siles E, Cañuelo A (2019) Tyrosol, a simple phenol from EVOO, targets multiple pathogenic mechanisms of neurodegeneration in a C. elegans model of Parkinson's disease. Neurobiol Aging 82, 60-68. | no |
1000 µM | whole life; start: A1 | OP50 | NGM agar | FUdR, penicillin, streptomycin | UA44 {baIn11 [dat-1p::α-synuclein, dat-1p::GFP]} | 20°C | up | | yes | A14 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Garcia-Moreno JC, Porta de la Riva M, Martínez-Lara E, Siles E, Cañuelo A (2019) Tyrosol, a simple phenol from EVOO, targets multiple pathogenic mechanisms of neurodegeneration in a C. elegans model of Parkinson's disease. Neurobiol Aging 82, 60-68. | no |
1000 µM | whole life; start: L1 | OP50 | NGM agar | FUdR, penicillin, streptomycin | NL5901 {pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Garcia-Moreno JC, Porta de la Riva M, Martínez-Lara E, Siles E, Cañuelo A (2019) Tyrosol, a simple phenol from EVOO, targets multiple pathogenic mechanisms of neurodegeneration in a C. elegans model of Parkinson's disease. Neurobiol Aging 82, 60-68. | no |