Treatment | Uncaria tomentosa, aqueous leaf extract | physical function | physiological function | healthspan biomarkers | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | body length / growth rate | | | | oxidative stress resistance | | | | ROS level | | | | activity / quantity / expression of HSPs | | | | activity / quantity / expression of SODs | | | | activity / quantity / expression of GSTs | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | | | | | | |
10 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | none | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
20 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | down | | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
40 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | up | | yes | A1 | up | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | -25.58 | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
80 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | none | | no | A1 | up | 76.23 | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | none | | no | A1 | up | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
40 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | up | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | up | 15.2 | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
80 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | up | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | none | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | up | | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
40 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | down | | no | A2 | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
80 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | down | -12.08 | yes | A2; no change under juglone-induced stress | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | none | | no | A2 | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
40 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | down | -32.57 | yes | A2; pre-exposed to juglone | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
80 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | down | | yes | A2; pre-exposed to juglone | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | down | -63.31 | yes | A2; pre-exposed to juglone | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
40 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | up | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
80 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | up | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | up | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | -27.06 | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
40 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | down | -41.77 | yes | A2; pre-exposed to juglone | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
80 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | down | -41.35 | yes | A2; pre-exposed to juglone | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | down | | no | A2; pre-exposed to juglone | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |