Treatment | Wheat gluten hydrolysate | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||||
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CID (PubChem) | 135305709 | ChEMBL | locomotion on solid media | body length / growth rate | number of offspring | heat stress resistance | oxidative stress resistance | autofluorescence (age pigment / lipofuscin) | ROS level | mean lifespan | ||||||||||||||||||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
0.1 mg/ml | whole life; start: L4 | OP50 | NGM agar | Gentamycin | N2 (wild type) | 20°C | none | no | A1; endpoints: body bends and head thrashes | none | no | A1 | none | no | down | yes | A12; unchanged in A1 | none | no | A1; measured via H2DCF-DA | up | yes | Zhang W, Lv T, Li M, Wu Q, Yang L, Liu H, Sun D, Sun L, Zhuang Z, Wang D (2013) Beneficial effects of wheat gluten hydrolysate to extend lifespan and induce stress resistance in nematode Caenorhabditis elegans. PLoS One 8, e74553. | |||||||||||||||||||||
0.5 mg/ml | whole life; start: L4 | OP50 | NGM agar | Gentamycin | N2 (wild type) | 20°C | none | no | A1; endpoints: body bends and head thrashes | none | no | A1 | none | no | down | yes | A12; unchanged in A1 | none | no | A1; measured via H2DCF-DA | up | yes | Zhang W, Lv T, Li M, Wu Q, Yang L, Liu H, Sun D, Sun L, Zhuang Z, Wang D (2013) Beneficial effects of wheat gluten hydrolysate to extend lifespan and induce stress resistance in nematode Caenorhabditis elegans. PLoS One 8, e74553. | |||||||||||||||||||||
1 mg/ml | whole life; start: L4 | OP50 | NGM agar | Gentamycin | N2 (wild type) | 20°C | up | yes | A4, A8, A12, A16, and A20; unchanged in A1; endpoints: body bends and head thrashes | none | no | A1 | none | no | up | yes | A2; Stressor: 35°C for 16 h; endpoint: mean lifespan | up | yes | A2; Stressor: 2 mM paraquat for 6 h; endpoint: mean lifespan | down | yes | A12; unchanged in A1 | down | yes | A2; pre-exposure to 2 mM paraquat for 6 h; unchanged ROS-Level without paraquat exposure in A1; measured via H2DCF-DA | up | yes | Zhang W, Lv T, Li M, Wu Q, Yang L, Liu H, Sun D, Sun L, Zhuang Z, Wang D (2013) Beneficial effects of wheat gluten hydrolysate to extend lifespan and induce stress resistance in nematode Caenorhabditis elegans. PLoS One 8, e74553. |