Treatment | ß-Caryophyllene | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 5281515 | ChEMBL | | | | | pharyngeal pumping | | | | body length / growth rate | | | | number of offspring | | | | heat stress resistance | | | | oxidative stress resistance | | | | autofluorescence (age pigment / lipofuscin) | | | | ROS level | | | | activity / quantity / expression of SODs | | | | activity / quantity / expression of SKN-1 | | | | activity / quantity / expression of GSTs | | | | translocation of DAF-16 to nucleus | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
25 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 11,2 | yes | mean from 3 individual trials | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 22,2 | yes | mean from 3 individual trials | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
100 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 14,9 | yes | mean from 3 individual trials | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50, heat-inactivated | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 7,2 | yes | mean from 2 individual trials | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 12,2 | yes | mean from 2 individual trials | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | TK22 [mev-1(kn1)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 13,1 | yes | mean from 3 individual trials | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | DA1116 [eat-2(ad1116)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 2,5 | variable | mean from 2 individual trials; 1 of 2 trials is significant | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | GR1307 [daf-16(mgDf50)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 6,5 | yes | mean from 3 individual trials; 1 of 3 trials was not significant | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | VC199 [sir-2.1(ok434)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | variable | variable | no | 2 individual trials | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -1,3 | no | mean from 3 individual trials | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | down | | yes | A5 & A10 | down | | variable | A1, A2, A5, A10 | none | | no | | up | 16 | yes | A2; stressor: 35°C for 7h; endpoint: survival; also significant changes by using 35°C for 4h | up | 34 | yes | A2; stressor: 250 µM juglone for 5 h; endpoint: survival | down | -20 | yes | A4; blue fluorescence | down | | yes | A4; measurement via DCF-DA in live and dead worms | | | | | | | | | | | | | | | | | | | | | A2; up-regulation of daf-16, skn-1, gst-4, gst-7, sir-2.1, sod-3, sod-2, hsp-70, daf-9, pha-4, hsf-1 | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | no | A1 | | | | | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A1 | | | | | | | | | | | | | | | | | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A1 | | | | | | | | | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | | LD1 {ldIs7 [skn-1b/c::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A1 | | | | | | | | | | | | | | | | | Pant A, Mishra V, Saikia SK, Shukla V, Asthana J, Akhoon BA, Pandey R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, 81-95. | no |