| Treatment | caffeine | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
|---|
| CID (PubChem) | | ChEMBL | | | | | locomotion on solid media | | | | oxidative stress resistance | | | | ROS level | | | | quantity of β-Amyloid | | | | activity of acetylcholinesterase | | | | mean lifespan | | | | | | | | | |
|---|
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | | | | | | |
| 200 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | up | | no | A1; stressor: 150 µM juglone for 7.5h; endpoint: survival | down | -23.3 | yes | A1; measured via H2DCF-DA; similar change when pre-exposed to juglone | | | | | | | | | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 400 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | up | | yes | A1; stressor: 150 µM juglone for 7.5h; endpoint: survival | down | -58.9 | yes | A1; measured via H2DCF-DA; similar change when pre-exposed to juglone | | | | | | | | | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 200 µM | whole life; start: L1 | OP50 | NGM agar | | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | up | 11 | yes | A1; temperature upshift to 35°C during measurement; paralysis assay | | | | | none | | no | A1; measured via H2DCF-DA | down | -40.6 | yes | A1; measured via qPCR | down | -38.4 | yes | A1 | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 400 µM | whole life; start: L1 | OP50 | NGM agar | | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | up | 21 | yes | A1; temperature upshift to 35°C during measurement; paralysis assay | | | | | none | | no | A1; measured via H2DCF-DA | down | -43.2 | yes | A1; measured via qPCR | down | -34.6 | yes | A1 | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 200 µM | whole life; start: L1 | HT115 (DE3) | NGM agar | Ampicillin, IPTG | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | up | 5.6 | yes | A1; temperature upshift to 35°C during measurement; paralysis assay | | | | | | | | | | | | | | | | | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 400 µM | whole life; start: L1 | HT115 (DE3) | NGM agar | Ampicillin, IPTG | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | up | 9.6 | yes | A1; temperature upshift to 35°C during measurement; paralysis assay | | | | | | | | | | | | | | | | | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against daf-16 | NGM agar | Ampicillin, IPTG | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | up | 6.2 | yes | A1; temperature upshift to 35°C during measurement; paralysis assay | | | | | | | | | | | | | | | | | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 400 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against daf-16 | NGM agar | Ampicillin, IPTG | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | up | 13.8 | yes | A1; temperature upshift to 35°C during measurement; paralysis assay | | | | | | | | | | | | | | | | | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against hsf-1 | NGM agar | Ampicillin, IPTG | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | down | -1.1 | no | A1; temperature upshift to 35°C during measurement; paralysis assay | | | | | | | | | | | | | | | | | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 400 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against hsf-1 | NGM agar | Ampicillin, IPTG | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | down | -0.7 | no | A1; temperature upshift to 35°C during measurement; paralysis assay | | | | | | | | | | | | | | | | | | | | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 200 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 25.9 | yes | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 400 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 31.2 | yes | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 200 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | | | | | | | | | | | | | | | | | | | | | up | 10 | yes | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |
| 400 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | | | | | | | | | | | | | | | | | | | | | up | 14.7 | yes | | | | | | Machado ML, Arantes LP, da Silveira TL, Zamberlan DC, Cordeiro LM, Obetine FBB, da Silva AF, da Cruz IBM, Soares FAA, Oliveira RP (2019) Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans. Nutr Neurosci 1-13. | no |