| Treatment | Carnosol | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| CID (PubChem) | ChEMBL | locomotion on solid media | number of offspring | quantity of late reproduction | oxidative stress resistance | heat stress resistance | ROS level | translocation of DAF-16 to nucleus | activity / quantity / expression of SODs | activity / quantity / expression of CTLs | triglyceride / fat / lipid content | autofluorescence (age pigment / lipofuscin) | activity / quantity / expression of GSH-PX | MDA level | mean lifespan | maximum lifespan | median lifespan | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | ||||||
| 60 µM | whole life; start: egg stage | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | A1; measured via H2DCF-DA | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 120 µM | whole life; start: egg stage | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | A1; measured via H2DCF-DA | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | N2 (wild type) | 20°C | up | yes | A14 & A6; unchanged at A10; endpoints: number of body bends; unchanged for head thrashes | down | no | down | yes | up | 21 | yes | A3; stressor: 10 mM paraquat; endpoint: mean survival | up | 9 | yes | A1; stressor: 35°C; endpoint: mean survival | down | -76 | yes | A1; measured via H2DCF-DA; similar change when pre-exposed to paraquat | up | 42 | yes | A1 | up | 18 | yes | A1 | none | no | A6; Oil Red O staining | down | yes | A6, A10, A14; green fluorescence | up | 87 | yes | A1 | down | -21 | yes | A1 | up | 19 | yes | up | n.d. | up | n.d. | A1; upregulation of sod-3, sod-5, hsf-1, hsp-16.1, hsp-16.2 | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | ||||||||||||||||||||
| 240 µM | whole life; start: egg stage | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | A1; measured via H2DCF-DA | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 300 µM | whole life; start: egg stage | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | A1; measured via H2DCF-DA | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | GR1307 [daf-16(mgDf50)] | 20°C | up | 12 | yes | up | n.d. | up | n.d. | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | PS3551 [hsf-1(sy441)] | 20°C | none | no | none | n.d. | none | n.d. | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 15°C | up | 21 | yes | L2; temperature upshift to 25°C during measurement; paralysis assay | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 20°C | up | 14 | yes | A8; paralysis assay | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | up | yes | A6 | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | yes | A3 | Lin C, Zhang X, Su Z, Xiao J, Lv M, Cao Y, Chen Y (2019) Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 5958043. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||