Treatment | Echinacoside | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | | | | | | pharyngeal pumping | | | | number of offspring | | | | heat stress resistance | | | | oxidative stress resistance | | | | translocation of DAF-16 to nucleus | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
50 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 3,2 | no | | | | | | Wang, X., Zhang, J., Lu, L., & Zhou, L. (2015). The longevity effect of echinacoside in Caenorhabditis elegans mediated through daf-16. Bioscience, biotechnology, and biochemistry, 79(10), 1676-1683. | |
150 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 7,6 | yes | | | | | | Wang, X., Zhang, J., Lu, L., & Zhou, L. (2015). The longevity effect of echinacoside in Caenorhabditis elegans mediated through daf-16. Bioscience, biotechnology, and biochemistry, 79(10), 1676-1683. | |
300 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 15,8 | yes | | | | | | Wang, X., Zhang, J., Lu, L., & Zhou, L. (2015). The longevity effect of echinacoside in Caenorhabditis elegans mediated through daf-16. Bioscience, biotechnology, and biochemistry, 79(10), 1676-1683. | |
600 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 9,5 | yes | | | | | | Wang, X., Zhang, J., Lu, L., & Zhou, L. (2015). The longevity effect of echinacoside in Caenorhabditis elegans mediated through daf-16. Bioscience, biotechnology, and biochemistry, 79(10), 1676-1683. | |
300 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | | N2 (wild type) | 20°C | none | | no | A4 | none | | no | | up | | yes | A3; stressor: 37°C for 3.5 h; endpoint: survival | up | 35 | yes | A2; stressor: 20 mM paraquat for 24h; endpoint: survival; percentage change refers to A6 | | | | | | | | | A4; upregulation of daf-16, ctl-1, hsp-16.2, sod-3 | | | DAF-16 | Wang, X., Zhang, J., Lu, L., & Zhou, L. (2015). The longevity effect of echinacoside in Caenorhabditis elegans mediated through daf-16. Bioscience, biotechnology, and biochemistry, 79(10), 1676-1683. | |
300 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | FUdR | GR1307 [daf-16(mgDf50)] | 20°C | | | | | | | | | | | | | | | | | | | | | down | -0,4 | no | | | | | | Wang, X., Zhang, J., Lu, L., & Zhou, L. (2015). The longevity effect of echinacoside in Caenorhabditis elegans mediated through daf-16. Bioscience, biotechnology, and biochemistry, 79(10), 1676-1683. | |
300 µM | 2 h; start: L4 | OP50, heat-inactivated | NGM agar | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | up | | yes | L4 | | | | | | | | | Wang, X., Zhang, J., Lu, L., & Zhou, L. (2015). The longevity effect of echinacoside in Caenorhabditis elegans mediated through daf-16. Bioscience, biotechnology, and biochemistry, 79(10), 1676-1683. | |