Treatment | Glutaredoxin from recombinant buckwheat | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 9501 | ChEMBL | | | | | pharyngeal pumping | | | | locomotion on solid media | | | | heat stress resistance | | | | oxidative stress resistance | | | | autofluorescence (age pigment / lipofuscin) | | | | ROS level | | | | activity / quantity / expression of CTLs | | | | activity / quantity / expression of SODs | | | | MDA level | | | | GSH level | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
1 µM | whole life; start: L4 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | 87,5 | yes | A10; +26.7% at A5 | up | 20,7 | yes | A10; +9% at A5 | up | | n.d. | A2; stressor: 35°C; endpoint: survival | up | | yes | A2; stressor: 240 µM juglone; endpoint: survival | | | | | down | | yes | A2; measured via DCF-DA | up | | yes | A2 | up | | yes | A2 | down | | yes | A2 | up | | yes | A2 | up | 8,5 | yes | | | | | | Li, F., Ma, X., Cui, X., Li, J., & Wang, Z. (2018). Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Experimental gerontology, 104, 86-97. | yes |
2 µM | whole life; start: L4 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | up | | n.d. | A2; stressor: 35°C; endpoint: survival | up | | yes | A2; stressor: 240 µM juglone; endpoint: survival | | | | | down | | yes | A2; measured via DCF-DA | up | | yes | A2 | up | | yes | A2 | down | | yes | A2 | up | | yes | A2 | up | 15,6 | yes | | | | | | Li, F., Ma, X., Cui, X., Li, J., & Wang, Z. (2018). Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Experimental gerontology, 104, 86-97. | yes |
4 µM | whole life; start: L4 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | up | | yes | A2; stressor: 35°C; endpoint: survival | up | | yes | A2; stressor: 240 µM juglone; endpoint: survival; similar results with 10 mM H2O2 | down | | yes | A10; green fluorescence | down | | yes | A2; with and without juglone-stress; measured via DCF-DA | up | | yes | A2 | up | | yes | A2 | down | | yes | A2 | up | | yes | A2 | up | 22 | yes | | A2; upregulation of hsf-1, hsp-16.2, hsp-70; downregulation of skn-1 | | | | Li, F., Ma, X., Cui, X., Li, J., & Wang, Z. (2018). Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Experimental gerontology, 104, 86-97. | yes |
4 µM | whole life; start: L4 | OP50 | NGM agar | | PS3551 [hsf-1(sy441)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | Li, F., Ma, X., Cui, X., Li, J., & Wang, Z. (2018). Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Experimental gerontology, 104, 86-97. | yes |
4 µM | whole life; start: L4 | OP50 | NGM agar | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | | | | | | Li, F., Ma, X., Cui, X., Li, J., & Wang, Z. (2018). Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Experimental gerontology, 104, 86-97. | yes |
4 µM | whole life; start: L4 | OP50 | NGM agar | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | | | | | | Li, F., Ma, X., Cui, X., Li, J., & Wang, Z. (2018). Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Experimental gerontology, 104, 86-97. | yes |
4 µM | whole life; start: L4 | OP50 | NGM agar | | CB1370 [daf-2(e1370)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | | | | | | Li, F., Ma, X., Cui, X., Li, J., & Wang, Z. (2018). Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Experimental gerontology, 104, 86-97. | yes |
4 µM | whole life; start: L4 | OP50 | NGM agar | | TJ1052 [age-1(hx546)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | | | | | | Li, F., Ma, X., Cui, X., Li, J., & Wang, Z. (2018). Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Experimental gerontology, 104, 86-97. | yes |