Treatment | metformin | physical function | reproductive function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | pharyngeal pumping | | | | locomotion in liquid media | | | | speed of larval development | | | | quantity of early reproduction | | | | quantity of late reproduction | | | | translocation of SKN-1 to nucleus | | | | triglyceride / fat / lipid content | | | | autofluorescence (age pigment / lipofuscin) | | | | activity / quantity / expression of GSTs | | | | mean lifespan | | | | median lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | | | | | | |
25 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 18.33 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 33.28 | yes | | | | | | | | A4; up-regulation of: Methylene THF, 5-Methyl THF, S-adenosylhomocysteine; down-regulation of: S-adenosylmethionine | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
100 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 3.45 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
25 mM | whole life; start: A8 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 08.03 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: A8 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 1.51 | no | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
100 mM | whole life; start: A8 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -15.98 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: 2 generations before | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 19.33 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 23.20 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 27.40 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50, UV-inactivated | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -15.69 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | none | axenic medium plates | FUDR, Carbenicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -40.53 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
2 mM | whole life; start: L4 | none | axenic medium plates | FUDR, Carbenicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -7.26 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
10 mM | whole life; start: L4 | none | axenic medium plates | FUDR, Carbenicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -16.38 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR, Carbenicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 29.90 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | Bacillus subtilis | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 9.44 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | metformin pretreated OP50 | NGM agar | FUDR, Carbenicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 33.30 | yes | metformin-free NGM plates | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50-R26 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 38.63 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR, pH = 6 | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 33.92 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR, pH = 5.5 | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -10.56 | no | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR, pH = 5 | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -17.42 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | BL21-Gold(DE3) | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -29.92 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50-MR (metformin resistant) | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -36.89 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
100 mM | whole life; start: L4 | OP50-MR (metformin resistant) | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -23.19 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | HT115 (DE3) | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -16.87 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | MG1655 bacteria | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -20.13 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | GD1 ubiG, GD1::pAHG (ubiG+) | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 19.85 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | CS180 bacteria | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 56.65 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | CS2429 bacteria | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -15.55 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | HB101 bacteria | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 1.12 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50-TmpR (Trimethoprim resistant bacteria) | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 32.46 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | RB755 [metr-1(ok521)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 67.17 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | RB2240 [sams-1(ok3033)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -38.24 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
2 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | GA1001 [aak-2(ok524)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 2.21 | no | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
10 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | GA1001 [aak-2(ok524)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -4.67 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | GA1001 [aak-2(ok524)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -8.78 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | EU31 [skn-1(zu135)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -15.53 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | metformin pretreated OP50 | NGM agar | FUDR, Carbenicillin | GA1001 [aak-2(ok524)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 28.66 | yes | metformin-free NGM plates | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | metformin pretreated OP50 | NGM agar | FUDR, Carbenicillin | EU31 [skn-1(zu135)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 20.55 | yes | metformin-free NGM plates | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR, 0.25% D-glucose | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -19.68 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | FUDR, 1% D-glucose | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -10.85 | yes | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L1 | OP50 | NGM agar | | EU31 [skn-1(zu135)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | down | | n.d. | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | none | | n.d. | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L1 | HT115 (DE3) | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 30 | yes | L4; expression of gst-4; measured via fluorescence | | | | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
50 mM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 29 | yes | L4; expression of gst-4; measured via fluorescence | | | | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
1 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | none | | no | A1 | | | | | | | | | none | | no | | none | | no | | | | | | down | | no | A2; Nile Red staining | down | | no | A2 | | | | | | | | | up | 13.33 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
10 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | none | | no | A1 | | | | | | | | | none | | no | | none | | no | | | | | | down | | no | A2; Nile Red staining | down | | no | A2 | | | | | | | | | up | 13.33 | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | none | | no | A1 | up | | yes | A7 & A12; no change at A2 | | | | | down | | yes | | up | | yes | | | | | | down | | yes | A2; Nile Red staining | down | | yes | A2 | | | | | | | | | up | 26.67 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
100 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | n.d. | A2 | | | | | | | | | | | | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 9.1 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
1 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | GR1307 [daf-16(mgDf50)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
10 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | GR1307 [daf-16(mgDf50)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | GR1307 [daf-16(mgDf50)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 15.38 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | TJ1052 [age-1(hx546)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 36.36 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
1 mM | whole life; start: A1 | OP50 | NGM agar | | CB1370 [daf-2(e1370)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 14.81 | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
10 mM | whole life; start: A1 | OP50 | NGM agar | | CB1370 [daf-2(e1370)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: A1 | OP50 | NGM agar | | CB1370 [daf-2(e1370)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 22.22 | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
1 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | DA1116 [eat-2(ad1116)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
10 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | DA1116 [eat-2(ad1116)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -9.52 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | DA1116 [eat-2(ad1116)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -9.52 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
1 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | RB754 [aak-2(ok524)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A2; Nile Red staining | down | | no | A2 | | | | | | | | | down | -5.56 | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
10 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | RB754 [aak-2(ok524)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A2; Nile Red staining | down | | no | A2 | | | | | | | | | down | -11.11 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | RB754 [aak-2(ok524)] | 20°C | | | | | down | | yes | A2 & A7 | | | | | | | | | | | | | | | | | down | | no | A2; Nile Red staining | down | | yes | A2 | | | | | | | | | down | -11.11 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
1 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | MR507 [aak-2(rr48)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -6.25 | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
10 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | MR507 [aak-2(rr48)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -6.25 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | MR507 [aak-2(rr48)] | 20°C | | | | | down | | yes | A2 & A7 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -18.75 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | | KK184 [par-4(it47)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: L4 | OP50 | NGM agar | | KK300 [par-4(it57)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -10 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | EU31 [skn-1(zu135)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | LD1 {ldIs7 [skn-1b/c::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | up | | yes | L4 | | | | | | | | | | | | | | | | | up | 25 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | LG348 {skn-1(zu135)/nT1[qIs51];geIs9[gpa-4p::skn-1b::gfp; rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -18.75 | yes | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | LG357 {skn-1(zu135)/nT1[qIs51];geIs10[ges-1p::skn-1c::gfp; rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 15.38 | no | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |
50 mM | whole life; start: eggs/L1 | OP50 | NGM agar | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A2 | | | | | | | | | | | | | Onken B, Driscoll M (2010) Metformin induces a dietary restriction-like state and the oxidative stress response to extend C. elegans Healthspan via AMPK, LKB1, and SKN-1. PLoS One 5, e8758. | no |