Treatment | Myricetintrimethylether | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 5481248 | ChEMBL | | | | | pharyngeal pumping | | | | body length / growth rate | | | | number of offspring | | | | vertex (progeny per day) | | | | heat stress resistance | | | | autofluorescence (age pigment / lipofuscin) | | | | ROS level | | | | translocation of DAF-16 to nucleus | | | | median lifespan | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin, | N2 (wild type) | 20°C | none | | no | A1 | none | | no | A4 | none | | no | | none | | no | | up | 20,5 | yes | A2; stressor: 37°C; endpoint: mean survival via SYTOX-staining; similar results in median survival | | | | | down | -19,8 | yes | A2; 37°C-stress prior measurement: measurement via DCF-DA | | | | | | | | | | | | | | | A1 | DAF-16 | Büchter, C., Ackermann, D., Honnen, S., Arnold, N., Havermann, S., Koch, K., & Wätjen, W. (2015). Methylated derivatives of myricetin enhance life span in Caenorhabditis elegans dependent on the transcription factor DAF-16. Food & function, 6(10), 3383-3392. | |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin, | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | | | | | up | 26,3 | yes | A2; stressor: 37°C; endpoint: mean survival via SYTOX-staining; similar results in median survival | | | | | down | | no | A2; 37°C-stress prior measurement: measurement via DCF-DA | | | | | | | | | | | | | | | | | Büchter, C., Ackermann, D., Honnen, S., Arnold, N., Havermann, S., Koch, K., & Wätjen, W. (2015). Methylated derivatives of myricetin enhance life span in Caenorhabditis elegans dependent on the transcription factor DAF-16. Food & function, 6(10), 3383-3392. | |
100 µM | start: L4; stop: A3 | OP50-1 | liquid NGM | BSA, streptomycin, | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | down | -55,6 | yes | A4 | | | | | | | | | | | | | | | | | | | | | Büchter, C., Ackermann, D., Honnen, S., Arnold, N., Havermann, S., Koch, K., & Wätjen, W. (2015). Methylated derivatives of myricetin enhance life span in Caenorhabditis elegans dependent on the transcription factor DAF-16. Food & function, 6(10), 3383-3392. | |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin, | N2 (wild type) | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 100 | n.d. | | up | 53,6 | yes | | | | | | Büchter, C., Ackermann, D., Honnen, S., Arnold, N., Havermann, S., Koch, K., & Wätjen, W. (2015). Methylated derivatives of myricetin enhance life span in Caenorhabditis elegans dependent on the transcription factor DAF-16. Food & function, 6(10), 3383-3392. | |
100 µM | whole life; start: L4 | OP50-1 | liquid NGM | BSA, streptomycin, | CF1038 [daf-16(mu86)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | n.d. | | up | 1,9 | no | | | | | | Büchter, C., Ackermann, D., Honnen, S., Arnold, N., Havermann, S., Koch, K., & Wätjen, W. (2015). Methylated derivatives of myricetin enhance life span in Caenorhabditis elegans dependent on the transcription factor DAF-16. Food & function, 6(10), 3383-3392. | |
100 µM | 1 h; start: L4/A1 | OP50-1 | liquid NGM | BSA, streptomycin, | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 60 | yes | L4/A1 | | | | | | | | | | | | | Büchter, C., Ackermann, D., Honnen, S., Arnold, N., Havermann, S., Koch, K., & Wätjen, W. (2015). Methylated derivatives of myricetin enhance life span in Caenorhabditis elegans dependent on the transcription factor DAF-16. Food & function, 6(10), 3383-3392. | |