| Treatment | Raspberry, acetone extract | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | |||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CID (PubChem) | ChEMBL | locomotion on solid media | body length / growth rate | heat stress resistance | UV stress resistance | translocation of DAF-16 to nucleus | autofluorescence (age pigment / lipofuscin) | mean lifespan | maximum lifespan | |||||||||||||||||||||||||||||||||||
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | ||||||
| 20 mg/ml | whole life; start: A1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | up | n.d. | A14, A18; unchanged at A8 | none | no | A5 | up | 14.5 | yes | A5; stressor: 35°C; endpoint: mean survival | up | 17 | no | A5; stressor: UV-B; endpoint: mean survival | down | -18.7 | yes | A5; green fluorescence | up | 13.6 | yes | up | 3.8 | n.d. | A5; downregulation of daf-2, age-1; upregulation of daf-16, skn-1, jnk-1, hsp-16.2 | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | |||||||||||
| 40 mg/ml | whole life; start: A1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | up | n.d. | A14, A18; unchanged at A8 | none | no | A5 | up | 29.1 | yes | A5; stressor: 35°C; endpoint: mean survival | up | 34.4 | yes | A5; stressor: UV-B; endpoint: mean survival | down | -35.6 | yes | A5; green fluorescence | up | 22.9 | yes | up | 7.7 | n.d. | A5; downregulation of daf-2, age-1, akt-2; upregulation of daf-16, skn-1, jnk-1, hsp-16.3, sir-2.1 | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | |||||||||||
| 80 mg/ml | whole life; start: A1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | up | n.d. | A14, A18; unchanged at A8 | none | no | A5 | up | 37.2 | yes | A5; stressor: 35°C; endpoint: mean survival | up | 41.1 | yes | A5; stressor: UV-B; endpoint: mean survival | down | -47.1 | yes | A5; green fluorescence | up | 29.7 | yes | up | 7.7 | n.d. | A5; downregulation of daf-2, age-1, akt-2; upregulation of daf-16, skn-1, jnk-1, hsp-16.3, sir-2.1 | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | |||||||||||
| 20 mg/ml | whole life; start: A1 | OP50 | NGM agar | FUdR | CB1370 [daf-2(e1370)] | 20°C | up | 1.8 | no | up | 3 | n.d. | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | ||||||||||||||||||||||||||||||
| 40 mg/ml | whole life; start: A1 | OP50 | NGM agar | FUdR | CB1370 [daf-2(e1370)] | 20°C | none | no | down | -3 | n.d. | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | |||||||||||||||||||||||||||||||
| 80 mg/ml | whole life; start: A1 | OP50 | NGM agar | FUdR | CB1370 [daf-2(e1370)] | 20°C | none | no | none | n.d. | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | ||||||||||||||||||||||||||||||||
| 20 mg/ml | whole life; start: A1 | HT115 (DE3) with RNAi-vector against daf-16 | NGM agar | FUdR | N2 (wild type) | 20°C | up | 1.2 | no | none | n.d. | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | |||||||||||||||||||||||||||||||
| 40 mg/ml | whole life; start: A1 | HT115 (DE3) with RNAi-vector against daf-16 | NGM agar | FUdR | N2 (wild type) | 20°C | up | 5.9 | no | up | 5.9 | n.d. | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | ||||||||||||||||||||||||||||||
| 80 mg/ml | whole life; start: A1 | HT115 (DE3) with RNAi-vector against daf-16 | NGM agar | FUdR | N2 (wild type) | 20°C | up | 3.6 | no | none | n.d. | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | |||||||||||||||||||||||||||||||
| 20 mg/ml | whole life; start: A1 | OP50 | NGM agar | FUdR | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | A5 | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | ||||||||||||||||||||||||||||||||||
| 40 mg/ml | whole life; start: A1 | OP50 | NGM agar | FUdR | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | A5 | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | ||||||||||||||||||||||||||||||||||
| 80 mg/ml | whole life; start: A1 | OP50 | NGM agar | FUdR | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | A5 | Song B, Zheng B, Li T, Liu RH (2020) Raspberry extract promoted longevity and stress tolerance via the insulin/IGF signaling pathway and DAF-16 in Caenorhabditis elegans. Food Funct 11, 3598-3609. | no | ||||||||||||||||||||||||||||||||||