| Treatment | Tambulin | cognitive function | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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| CID (PubChem) | ChEMBL | chemotaxis | pharyngeal pumping | locomotion in liquid media | oxidative stress resistance | ROS level | activity / quantity / expression of SODs | quantity of α-synuclein | quantity of carbonylated proteins | triglyceride / fat / lipid content | autofluorescence (age pigment / lipofuscin) | quantity of apoptotic cells | mean lifespan | survival at a given time point | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | ||||||
| 5 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | up | no | none | no | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| 25 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | up | 11.7 | no | stressor: 250 μM juglone; endpoint: survival | down | -30.5 | no | none | no | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | ||||||||||||||||||||||||||||||||||||||||||||||||
| 50 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | up | yes | repellent: 1-nonanol; endpoint: speed of response | up | 7.5 | yes | A3 | up | yes | endpoints: body bends and head thrashes | up | 50 | yes | stressor: 250 μM juglone; endpoint: survival | down | -59 | yes | measured via H2DCF-DA | down | -15.6 | yes | A1; Oil O Red staining | down | -29.2 | yes | A5; blue fluorescence | down | -24.4 | yes | A1 | none | no | A1; upregulation of daf-16, sod-1, sod-3, ctl-2; downregulation of sod-2 | A1; increased dopamine level | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||
| 100 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | up | 50 | yes | stressor: 250 μM juglone; endpoint: survival | down | -82.7 | yes | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | ||||||||||||||||||||||||||||||||||||||||||||||||||
| 250 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 500 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 1000 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 25 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | down | -5.4 | no | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| 50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | down | -42.6 | yes | A5 | up | 16.8 | yes | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||
| 100 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | down | -7.8 | no | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| 50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | unknown strain [daf-16 deletion mutant] | 20°C | up | 3.5 | yes | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| 50 µM | whole life; start: L1 | OP50 | NGM agar | NL5901 {pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]} | 20°C | up | yes | repellent: 1-nonanol; endpoint: speed of response | up | 8.8 | yes | A3 | up | yes | endpoints: body bends and head thrashes | down | -19.4 | yes | A1 | down | -13.6 | yes | A1; Oil O Red staining | down | -12.3 | yes | A1 | A1: upregulation of lagr-1, pdr-1, lrk-1, ubc-12, ymel-1 | A1; increased dopamine level | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||
| 50 µM | whole life; start: L1 | OP50 | NGM agar | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | up | 46.9 | yes | A1 | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| 50 µM | whole life; start: L1 | OP50 | NGM agar | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | Pandey T, Sammi SR, Nooreen Z, Mishra A, Ahmad A, Bhatta RS, Pandey R (2019) Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp Gerontol 120, 50-61. | yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||