Treatment | Alpinia zerumbet Leaf (Phenol-rich fraction) | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | heat stress resistance | | | | oxidative stress resistance | | | | activity / quantity / expression of HSPs | | | | activity / quantity / expression of SODs | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
2.5 µg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | 8.3 | yes | A5; Stressor: 35°C; endpoint: mean lifespan; median lifespan is also significantly increased | up | 80.6 | yes | A2; Stressor: 0.5 mM H2O2 for 8 h | | | | | | | | | | | | | | | | | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |
5.0 µg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | 13.45 | yes | A5; Stressor: 35°C; endpoint: mean lifespan; median lifespan is also significantly increased | up | 93.7 | yes | A2; Stressor: 0.5 mM H2O2 for 8 h | | | | | | | | | | | | | | | | | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |
10.0 µg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | 16.2 | yes | A5; Stressor: 35°C; endpoint: mean lifespan; median lifespan is also significantly increased | up | 112.8 | yes | A2; Stressor: 0.5 mM H2O2 for 8 h | | | | | | | | | | | | | | | | SOD-3 and HSP-16.2 | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |
25 µg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | up | 4.22 | yes | | | | | | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |
50 µg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | up | 20.77 | yes | | | | | | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |
100 µg/ml | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | up | 45.49 | yes | | | | | | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |
10.0 µg/ml | whole life; start: L1 | OP50 | NGM agar | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | up | 143.00 | n.d. | A1; measured via fluorescence; up-regulation also visible via RT-PCR | | | | | | | | | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |
10.0 µg/ml | whole life; start: L1 | OP50 | NGM agar | | CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | up | 150.00 | yes | A1; 12 h before measurement: heat shock at 35°C for 2 h; measured via fluorescence; up-regulation also visible via RT-PCR | | | | | | | | | | | | | Upadhyay A, Chompoo J, Taira N, Fukuta M, Tawata S (2013) Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci Biotechnol Biochem 77, 217-223. | |