Treatment | Anacardium occidentale, methanol extract | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | pharyngeal pumping | | | | body length / growth rate | | | | number of offspring | | | | oxidative stress resistance | | | | ROS level | | | | activity / quantity / expression of HSPs | | | | translocation of DAF-16 to nucleus | | | | activity / quantity / expression of SODs | | | | translocation of SKN-1 to nucleus | | | | autofluorescence (age pigment / lipofuscin) | | | | activity / quantity / expression of GSTs | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | | | | | | |
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | up | 40.89 | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | -57.22 | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | up | 45.48 | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | -58.1 | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | up | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | up | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | down | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | down | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | up | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | none | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | none | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | down | -78.04 | yes | A2; pre-exposed to juglone | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | down | -68.27 | yes | A2; pre-exposed to juglone | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | down | -22.32 | yes | A2; pre-exposed to juglone | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 27.37 | yes | A2 | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 29.11 | yes | A2 | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 24.87 | yes | A2 | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | up | 49.68 | yes | A2 | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | up | 58.74 | yes | A2 | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | up | 68.17 | yes | A2 | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 27.38 | yes | A2; pre-exposed to juglone | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 23.8 | yes | A2; pre-exposed to juglone | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 50.19 | yes | A2; pre-exposed to juglone | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | LD001 {ldIs007 [skn-1p::SKN-1::gfp]; pRF4 [rol-6(su1006gf)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 28.49 | yes | A2 | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | LD001 {ldIs007 [skn-1p::SKN-1::gfp]; pRF4 [rol-6(su1006gf)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 22.55 | yes | A2 | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | LD001 {ldIs007 [skn-1p::SKN-1::gfp]; pRF4 [rol-6(su1006gf)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 24.1 | yes | A2 | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -12.25 | yes | A16; blue fluorescence | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -18.48 | yes | A16; blue fluorescence | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -8.39 | yes | A16; blue fluorescence | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L4 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | | yes | A5, A8, A10, A12 | none | | no | A1 | none | | no | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 3.36 | yes | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L4 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | | yes | A5, A8, A10; no significant change at A12 | none | | no | A1 | none | | no | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 2.45 | no | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L4 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | | yes | A5, A8, A10; no significant change at A12 | none | | no | A1 | none | | no | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 6.16 | no | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
1 µg/ml | whole life; start: L4 | OP50 | NGM agar | | TK22 [mev-1(kn1)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 03.02 | no | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
2.5 µg/ml | whole life; start: L4 | OP50 | NGM agar | | TK22 [mev-1(kn1)] | 20°C | | | | | | | | | | | | | | | | | | | | | 78.04% | 68.27% | and 22.32 | | | | | | | | | | | | | | | | | | | | | | up | 13.48 | no | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
5 µg/ml | whole life; start: L4 | OP50 | NGM agar | | TK22 [mev-1(kn1)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 1.23 | no | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |