Treatment | Arsenite | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 443495 | ChEMBL | | | | | locomotion in liquid media | | | | heat stress resistance | | | | oxidative stress resistance | | | | ROS level | | | | oxygen consumption rate (OCR) | | | | activity / quantity / expression of SODs | | | | activity / quantity / expression of CTLs | | | | GSH level | | | | mean lifespan | | | | maximum lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
0.01 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -2.49 | no | | up | 0.29 | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
0.1 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | N2 (wild type) | 20°C | up | | yes | adult animals; endpoint: distance moved | up | | yes | adult animals; Stressor: 37°C; endpoint: mean lifespan | up | | yes | A6; Stressor: 10 mM paraquat; endpoint: mean lifespan | variable | | yes | increased ROS-level in A1 and A2; decreased ROS-level in A5; endpoints: mitochondrial ROS formation and hydrogen peroxide formation | up | | yes | A5; unchanged in A1 and A2 | none | | no | A1, A2, and A5; SOD-activity measured via oxidation of WST-1 | up | | yes | A2, and A5; unchanged at A1; catalase-activity measured by formaldehyde formation | variable | | yes | decreased ratio in A1; increased ratio in A2 (not significant) and A5; | up | 9.54 | yes | | up | 11.50 | n.d. | | RNA deep sequencing in A2; list of differentially expressed genes in the supplement | | | DAF-16, SKN-1, TIN-9.1, and MTL-2 | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
1 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -3.32 | no | | down | -5.6 | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
10 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -9.13 | yes | | down | -17.40 | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
100 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -30.29 | yes | | down | -32.15 | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
1000 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -48.55 | yes | | down | -49.85 | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
0.1 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | 10 µM butylated hydroxyanisole; ampicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | none | | no | A1 and A5; endpoints: mitochondrial ROS formation and hydrogen peroxide formation | | | | | | | | | none | | no | A5; catalase-activity measured by formaldehyde formation | | | | | down | -2.44 | no | | none | | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
0.1 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | 1 mM N-acetyl-L-cysteine; ampicillin | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 1.67 | no | | none | | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
0.1 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -4.49 | yes | | down | -12.27 | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
0.1 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | unknown strain [daf-16(mgDf47)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -3.49 | yes | | down | -3.94 | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
0.1 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | VC2268 [tin-9.1(ok2194)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | up | 1.42 | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |
0.1 µM | whole life; start: L4 | OP50, heat-inactivated | NGM agar | ampicillin | VC128 [mtl-2(gk125)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 1.66 | no | | up | 8.62 | n.d. | | | | | | Schmeisser S, Schmeisser K, Weimer S, Groth M, Priebe S, Fazius E, Kuhlow D, Pick D, Einax JW, Guthke R, Platzer M, Zarse K, Ristow M (2013) Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell 12, 508-517. | |