150 µM | 3 h at A1 and 3 h at A2 | HT115 (DE3) | NGM agar | | CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]} | 20°C | none | | no | A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot | | | | | El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174. | |
300 µM | 3 h at A1 and 3 h at A2 | HT115 (DE3) | NGM agar | | CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]} | 20°C | none | | no | A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot | | | | | El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174. | |
600 µM | 3 h at A1 and 3 h at A2 | HT115 (DE3) | NGM agar | | CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]} | 20°C | none | | no | A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot | | | | | El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174. | |
900 µM | 3 h at A1 and 3 h at A2 | HT115 (DE3) | NGM agar | | CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]} | 20°C | none | | no | A2; pre-exposed to 33°C for 3 h; expression of hsp-16.2 measured via western blot | | | | | El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E (2014) A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13, 165-174. | |