Treatment | Epigallocatechin gallate (EGCG) | cognitive function | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 65064 | ChEMBL | | | | | chemotaxis | | | | pharyngeal pumping | | | | locomotion on solid media | | | | body length / growth rate | | | | number of offspring | | | | heat stress resistance | | | | oxidative stress resistance | | | | autofluorescence (age pigment / lipofuscin) | | | | ROS level | | | | activity / quantity / expression of SODs | | | | translocation of SKN-1 to nucleus | | | | activity / quantity / expression of HSPs | | | | activity / quantity / expression of HSPs | | | | activity / quantity / expression of GSTs | | | | quantity of β-Amyloid | | | | translocation of DAF-16 to nucleus | | | | mean lifespan | | | | maximum lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
10 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | none | | no | A2, A4, A6, A8 | | | | | | | | | | | | | | | | | | | | | | | | | down | | no | L4/A1, measurement via DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Brown, M. K., Evans, J. L., & Luo, Y. (2006). Beneficial effects of natural antioxidants EGCG and α-lipoic acid on life span and age-dependent behavioral declines in Caenorhabditis elegans. Pharmacology Biochemistry and Behavior, 85(3), 620-628. | |
25 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | | yes | A5; no significant changes at A1; attractant: diacetyl | up | | yes | A8; no significant changes in A2, A4, A6 | up | | yes | A16; no significant changes in A12 | | | | | | | | | | | | | | | | | | | | | down | | yes | L4/A1, measurement via DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 4,8 | no | | up | 4,76 | no | | | | | | Brown, M. K., Evans, J. L., & Luo, Y. (2006). Beneficial effects of natural antioxidants EGCG and α-lipoic acid on life span and age-dependent behavioral declines in Caenorhabditis elegans. Pharmacology Biochemistry and Behavior, 85(3), 620-628. | |
50 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | up | | yes | A6 & A8; no significant changes in A2 & A4 | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | L4/A1, measurement via DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Brown, M. K., Evans, J. L., & Luo, Y. (2006). Beneficial effects of natural antioxidants EGCG and α-lipoic acid on life span and age-dependent behavioral declines in Caenorhabditis elegans. Pharmacology Biochemistry and Behavior, 85(3), 620-628. | |
100 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | up | | yes | A4 & A6; no significant changes in A2 & A8 | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | L4/A1, measurement via DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Brown, M. K., Evans, J. L., & Luo, Y. (2006). Beneficial effects of natural antioxidants EGCG and α-lipoic acid on life span and age-dependent behavioral declines in Caenorhabditis elegans. Pharmacology Biochemistry and Behavior, 85(3), 620-628. | |
25 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | no | A5, measurement via DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Brown, M. K., Evans, J. L., & Luo, Y. (2006). Beneficial effects of natural antioxidants EGCG and α-lipoic acid on life span and age-dependent behavioral declines in Caenorhabditis elegans. Pharmacology Biochemistry and Behavior, 85(3), 620-628. | |
25 µM | whole life; start: L1 | OP50 | NGM agar | | GR1307 [daf-16(mgDf50)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | no | L4/A1, measurement via DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | 8,6 | no | | none | 0 | no | | | | | | Brown, M. K., Evans, J. L., & Luo, Y. (2006). Beneficial effects of natural antioxidants EGCG and α-lipoic acid on life span and age-dependent behavioral declines in Caenorhabditis elegans. Pharmacology Biochemistry and Behavior, 85(3), 620-628. | |
25 µM | whole life; start: egg stage | OP50 | NGM agar | | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 16°C | | | | | | | | | up | | no | L3; temperature shift to 23°C prior analysing paralysis | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Brown, M. K., Evans, J. L., & Luo, Y. (2006). Beneficial effects of natural antioxidants EGCG and α-lipoic acid on life span and age-dependent behavioral declines in Caenorhabditis elegans. Pharmacology Biochemistry and Behavior, 85(3), 620-628. | |
0.1 µg/ml | whole life; start: A1 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 13.1 | yes | A3; Stressor: 35°C; endpoint: mean lifespan; percentual change of maximum survival = 11.76 | up | 172.9 | yes | A3; Stressor: 500 µM juglone; endpoint: mean lifespan | | | | | down | | yes | A3; measured with H2DCF-DA; similar change during juglone treatment | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -1.8 | no | Also no life extension at 25°C (data not shown) | | | | | | | | sod-3, hsp-16.2, and skn-1 | Zhang L, Jie G, Zhang J, Zhao B (2009) Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress. Free Radic Biol Med 46, 414-421. | |
1 µg/ml | whole life; start: A1 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 8.0 | yes | A3; Stressor: 35°C; endpoint: mean lifespan; percentual change of maximum survival = 11.76 | up | 177.7 | yes | A3; Stressor: 500 µM juglone; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 1.7 | no | Also no life extension at 25°C (data not shown) | | | | | | | | | Zhang L, Jie G, Zhang J, Zhao B (2009) Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress. Free Radic Biol Med 46, 414-421. | |
10 µg/ml | whole life; start: A1 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | up | 11.8 | yes | A3; Stressor: 35°C; endpoint: mean lifespan; percentual change of maximum survival = 11.76 | up | 88.5 | yes | A3; Stressor: 500 µM juglone; endpoint: mean lifespan | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 1.8 | no | Also no life extension at 25°C (data not shown) | | | | | | | | | Zhang L, Jie G, Zhang J, Zhao B (2009) Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress. Free Radic Biol Med 46, 414-421. | |
0.1 µg/ml | whole life; start: A1 | OP50 | NGM agar | FUDR | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 49.00 | yes | A3; measured via fluorescence; similar changes also at A5, A7, A9 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Zhang L, Jie G, Zhang J, Zhao B (2009) Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress. Free Radic Biol Med 46, 414-421. | |
0.1 µg/ml | whole life; start: A1 | OP50 | NGM agar | FUDR | CL2070 {dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 11.9 | yes | A4; after heat shock (35°C for 1 h) and recovery (24h); measured via fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Zhang L, Jie G, Zhang J, Zhao B (2009) Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress. Free Radic Biol Med 46, 414-421. | |
220 µM | whole life; start: A1 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 10.14 | yes | | | | | | | | | | Abbas S, Wink M (2009) Epigallocatechin gallate from green tea (Camellia sinensis) increases lifespan and stress resistance in Caenorhabditis elegans. Planta Med 75, 216-221. doi: 10.1055/s-0028-1088378. | |
220 µM | whole life; start: L2/L3 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | up | 289.00 | yes | A1; Stressor: 80 µM juglone for 24 h | | | | | down | -32.57 | yes | A2; measured with DCF-DA assay | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Abbas S, Wink M (2009) Epigallocatechin gallate from green tea (Camellia sinensis) increases lifespan and stress resistance in Caenorhabditis elegans. Planta Med 75, 216-221. doi: 10.1055/s-0028-1088378. | |
220 µM | whole life; start: L2/L3 | OP50 | NGM agar | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -73.26 | yes | A2; after exposure to EGCG for 48 h and thereafter 20 µM juglone for 24 h; measured via fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Abbas S, Wink M (2009) Epigallocatechin gallate from green tea (Camellia sinensis) increases lifespan and stress resistance in Caenorhabditis elegans. Planta Med 75, 216-221. doi: 10.1055/s-0028-1088378. | |
220 µM | whole life; start: A1 | OP50 | NGM agar | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A15 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 14.27 | yes | | | | | | | | | | Abbas S, Wink M (2009) Epigallocatechin gallate from green tea (Camellia sinensis) increases lifespan and stress resistance in Caenorhabditis elegans. Planta Med 75, 216-221. doi: 10.1055/s-0028-1088378. | no |
55 µM | whole life; start: A1 | OP50 | NGM agar | | TK22 [mev-1(kn1)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 16.11 | yes | | | | | | | | | | Abbas S, Wink M (2009) Epigallocatechin gallate from green tea (Camellia sinensis) increases lifespan and stress resistance in Caenorhabditis elegans. Planta Med 75, 216-221. doi: 10.1055/s-0028-1088378. | no |
220 µM | start: L2/L3; stop: A1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A2; 20 µM juglone treatment for 24 h from A1 - A2; only slight (not significant) down regulation without juglone | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Abbas S, Wink M (2010) Epigallocatechin gallate inhibits beta amyloid oligomerization in Caenorhabditis elegans and affects the daf-2/insulin-like signaling pathway. Phytomedicine 17, 902-909. | no |
220 µM | whole life; start: L2/L3 | OP50 | liquid S-medium | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A15; green fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Abbas S, Wink M (2010) Epigallocatechin gallate inhibits beta amyloid oligomerization in Caenorhabditis elegans and affects the daf-2/insulin-like signaling pathway. Phytomedicine 17, 902-909. | no |
220 µM | 1 h; start: L2 | OP50 | liquid S-medium | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | | | | | | | | | | | | | Abbas S, Wink M (2010) Epigallocatechin gallate inhibits beta amyloid oligomerization in Caenorhabditis elegans and affects the daf-2/insulin-like signaling pathway. Phytomedicine 17, 902-909. | no |
220 µM | whole life; start: L4 | OP50 | liquid S-medium | | CL2006 {dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + pRF4]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A4; significance level was reached by fluorescent staining, but not by RT-PCR | | | | | | | | | | | | | | | | | Abbas S, Wink M (2010) Epigallocatechin gallate inhibits beta amyloid oligomerization in Caenorhabditis elegans and affects the daf-2/insulin-like signaling pathway. Phytomedicine 17, 902-909. | no |
200 µM | 48 h; start: unknown | OP50 | NGM agar | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | pre-treated with 20 µM juglone for 24h; measured via fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Su S, Wink M (2015) Natural lignans from Arctium lappa as antiaging agents in Caenorhabditis elegans. Phytochemistry 117, 340-50. | |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | down | | no | A1 | | | | | | | | | up | 92.35 | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | down | -43.36 | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | down | | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -23.82 | yes | A2 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -81.01 | yes | A2; pre-exposed to juglone | | | | | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | down | | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -35.02 | yes | A2; pre-exposed to juglone | | | | | | | | | | | | | | | | | | | | | | | | | Azevedo BC, Roxo M, Borges MC, Peixoto H, Crevelin EJ, Bertoni BW, Contini SHT, Lopes AA, França SC, Pereira AMS, Wink M (2019) Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules 24, 3299. | no |
25 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | up | 40.8 | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | down | | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | down | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | down | | no | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -61.91 | yes | A2; pre-exposed to juglone | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | A2 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 45.95 | yes | A2 | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | no | A2; pre-exposed to juglone | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | LD001 {ldIs007 [skn-1p::SKN-1::gfp]; pRF4 [rol-6(su1006gf)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A2 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -21.58 | yes | A16; blue fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: L4 | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | up | | yes | A5, A8, A10; no significant change at A12 | | | | | none | | no | A1 | none | | no | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Wink M, Tencomnao T (2019) Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxid Med Cell Longev 2019, 9012396. | no |
25 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | N2 (wild type) | 20°C | | | | | up | | yes | A5, A8, A10, A12; not significant at A12 | | | | | | | | | | | | | | | | | up | | yes | stressor: 80 μM juglone; endpoint: survival; 48h of compound treatment | | | | | down | -35.43 | yes | measured via H2DCF-DA; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
25 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | -61.91 | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
25 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
25 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | no | pre-exposed to juglone; 48h of compound treatment | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
25 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | yes | 72h of compound treatment | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
25 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | 72h of compound treatment | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |
25 µg/ml | whole life; start: n.d. | OP50 | NGM agar | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A16; blue fluorescence | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Duangjan C, Rangsinth P, Gu X, Zhang S, Wink M, Tencomnao T (2019) Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. Phytomedicine 64, 153061. | no |