| Treatment | Nobiletin | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| CID (PubChem) | ChEMBL | locomotion on solid media | body length / growth rate | number of offspring | oxidative stress resistance | heat stress resistance | UV stress resistance | ROS level | activity / quantity / expression of SODs | activity / quantity / expression of CTLs | autofluorescence (age pigment / lipofuscin) | MDA level | mean lifespan | maximum lifespan | median lifespan | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | ||||||
| 3.13 µM | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | up | yes | A18 & A22; unchanged at A14 | none | no | A5 | up | 11.6 | yes | A5; stressor: 10 mM paraquat; endpoint: mean survival | up | 18.2 | yes | A5; stressor: 35°C; endpoint: mean survival | up | 8.7 | no | A5; stressor: 254 nm of UV radiation at 1200 J/m2; endpoint: mean survival | down | yes | A5; measured via H2DCF-DA | up | yes | A5 | up | yes | A5 | down | -14.8 | yes | A5; blue fluorescence | down | yes | A5 | up | 8.7 | yes | up | n.d. | up | n.d. | Yang X, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Nobiletin Delays Aging and Enhances Stress Resistance of Caenorhabditis elegans. Int J Mol Sci 21, 341. | no | |||||||||||||||||||
| 6.25 µM | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | up | yes | A18 & A22; unchanged at A14 | none | no | A5 | up | 30.5 | yes | A5; stressor: 10 mM paraquat; endpoint: mean survival | up | 21.1 | yes | A5; stressor: 35°C; endpoint: mean survival | up | 20.5 | yes | A5; stressor: 254 nm of UV radiation at 1200 J/m2; endpoint: mean survival | down | yes | A5; measured via H2DCF-DA | up | yes | A5 | up | yes | A5 | down | -25.3 | yes | A5; blue fluorescence | down | yes | A5 | up | 14.9 | yes | up | n.d. | up | n.d. | A5; upregulation of sod-3, hsp-16.2 | Yang X, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Nobiletin Delays Aging and Enhances Stress Resistance of Caenorhabditis elegans. Int J Mol Sci 21, 341. | no | ||||||||||||||||||
| 12.5 µM | whole life; start: L4 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | up | yes | A14, A18, A22 | none | no | A5 | up | 47.8 | yes | A5; stressor: 10 mM paraquat; endpoint: mean survival | up | 30.6 | yes | A5; stressor: 35°C; endpoint: mean survival | up | 33.3 | yes | A5; stressor: 254 nm of UV radiation at 1200 J/m2; endpoint: mean survival | down | yes | A5; measured via H2DCF-DA | up | yes | A5 | up | yes | A5 | down | -31.4 | yes | A5; blue fluorescence | down | yes | A5 | up | 21 | yes | up | n.d. | up | n.d. | A5; upregulation of sod-3, hsp-16.2, gst-4, skn-1, sek-1, sir-2.1 | Yang X, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Nobiletin Delays Aging and Enhances Stress Resistance of Caenorhabditis elegans. Int J Mol Sci 21, 341. | no | ||||||||||||||||||
| 3.13 µM | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | Yang X, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Nobiletin Delays Aging and Enhances Stress Resistance of Caenorhabditis elegans. Int J Mol Sci 21, 341. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 6.25 µM | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | Yang X, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Nobiletin Delays Aging and Enhances Stress Resistance of Caenorhabditis elegans. Int J Mol Sci 21, 341. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 12.5 µM | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | none | no | Yang X, Wang H, Li T, Chen L, Zheng B, Liu RH (2020) Nobiletin Delays Aging and Enhances Stress Resistance of Caenorhabditis elegans. Int J Mol Sci 21, 341. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||