| Treatment | Rosmarinic acid | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| CID (PubChem) | ChEMBL | locomotion on solid media | number of offspring | quantity of early reproduction | quantity of late reproduction | oxidative stress resistance | heat stress resistance | ROS level | translocation of DAF-16 to nucleus | activity / quantity / expression of SODs | activity / quantity / expression of CTLs | triglyceride / fat / lipid content | autofluorescence (age pigment / lipofuscin) | GSH level | activity / quantity / expression of GSH-PX | MDA level | mean lifespan | maximum lifespan | median lifespan | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | ||||||
| 60 µM | whole life; start: egg stage | OP50 | NGM agar | N2 (wild type) | 20°C | up | yes | A7 & A11; unchanged at A3; endpoint: head thrashes; variable results for body bend frequency | up | 40 | yes | up | yes | up | yes | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 120 µM | whole life; start: egg stage | OP50 | NGM agar | N2 (wild type) | 20°C | up | yes | A3, A7, A11; similar results for body bend frequency | up | 49 | yes | up | yes | up | yes | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | N2 (wild type) | 20°C | up | yes | A3, A7, A11; similar results for body bend frequency | none | no | down | yes | up | yes | up | 41 | yes | A3; stressor: paraquat; endpoint: mean lifespan | up | 22 | yes | A1; stressor: 35°C; endpoint: mean lifespan | up | yes | A1; measured via H2DCF-DA; also significant after pre-exposure to paraquat | none | no | A1 | up | 58 | yes | A1 | down | yes | A3; Oil Red O staining & triglyceride content | down | -11 | yes | A11; similar at A3 & A7; green fluorescence | up | 95 | yes | A1 | up | 56 | yes | A1 | down | yes | A1 | up | 63 | yes | up | yes | up | yes | L4/A1; upregulation of sod-3, sod-5, ctl-1, daf-16, ins-18, skn-1, sek-1 | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | ||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 20°C | up | no | none | no | up | no | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | AM140 {rmIs132 [unc-54p::Q35::YFP]} | 20°C | none | no | paralysis assay | none | no | none | no | none | no | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | GR1307 [daf-16(mgDf50)] | 20°C | none | no | none | no | none | no | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | none | no | none | no | none | no | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | up | yes | A3 | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 25°C | none | no | from L1-L3: 15°C; paralysis assay | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 180 µM | whole life; start: egg stage | OP50 | NGM agar | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | none | no | A3 | Lin C, Xiao J, Xi Y, Zhang X, Zhong Q, Zheng H, Cao Y, Chen Y (2019) Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans. Biofactors 45, 774-787. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||