Treatment | Acacetin | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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CID (PubChem) | 5280442 | ChEMBL | pharyngeal pumping | body length / growth rate | vertex (progeny per day) | heat stress resistance | oxidative stress resistance | autofluorescence (age pigment / lipofuscin) | ROS level | activity / quantity / expression of SODs | activity / quantity / expression of CTLs | activity / quantity / expression of GSTs | quantity of α-synuclein | mean lifespan | maximum lifespan | |||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
5 µM | whole life; start: L1 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | down | no | A5 and A10 | none | no | A4 and A8 | up | 13.79 | yes | A2; stressor: 37°C for 4 h; endpoint: mean lifespan | up | yes | A2; stressor: 250 µM juglone; endpoint: survival | down | yes | A5; measured via H2DCF-DA | up | yes | A2; SOD activity | up | yes | A2; catalase activity | up | 9.86 | yes | up | n.d. | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no | |||||||||||||||||||||||||||||
25 µM | whole life; start: L1 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | down | no | A5 and A10 | variant | yes | slightly decreased in A4 and slightly increased in A8 | up | 32.41 | yes | A2; stressor: 37°C for 4 h; endpoint: mean lifespan | up | yes | A2; stressor: 250 µM juglone; endpoint: survival | down | yes | A4; blue fluorescence | down | yes | A5; measured via H2DCF-DA | up | yes | A2; SOD activity | up | yes | A2; catalase activity | up | 26.78 | yes | up | n.d. | up-regulation of sod-3, gst-4, hsp-16.2, and ctl-1 | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no | |||||||||||||||||||||||||
50 µM | whole life; start: L1 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | down | no | A5 and A10 | up | yes | A8; unchanged in A4 | up | 18.96 | yes | A2; stressor: 37°C for 4 h; endpoint: mean lifespan | up | yes | A2; stressor: 250 µM juglone; endpoint: survival | down | yes | A5; measured via H2DCF-DA | up | yes | A2; SOD activity | none | no | A2; catalase activity | up | 16.45 | yes | up | n.d. | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no | |||||||||||||||||||||||||||||
5 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
25 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
50 µM | whole life; start: L1 | OP50 | NGM agar | N2 (wild type) | 20°C | down | yes | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
25 µM | whole life; start: L1 | OP50 | NGM agar | FUDR | TK22 [mev-1(kn1)] | 20°C | up | 40.50 | yes | up | n.d. | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||
25 µM | whole life; start: L1 | OP50 | NGM agar | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | up | yes | A1; sod-3 expression measured via fluorescence | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
25 µM | whole life; start: L1 | OP50 | NGM agar | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | up | yes | A1; gst-4 expression measured via fluorescence | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
25 µM | whole life; start: L1 | OP50 | NGM agar | NL5901 {pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]} | 20°C | down | yes | A1; measured via fluorescence | Asthana J, Mishra BN, Pandey R (2016) Acacetin promotes healthy aging by altering stress response in Caenorhabditis elegans. Free Radic Res 50, 861-874. | no |