Treatment | Ayurvedic polyherbal extract (includes 6 herbs: B. aristata roots, C.rotundus rhizome, C. deodara stem bark, E. officinalisfruits, T. chebula fruits and T. bellirica fruits) | cognitive function | physical function | reproductive function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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CID (PubChem) | ChEMBL | chemotaxis | aldicarb resistance | pharyngeal pumping | locomotion on solid media | number of offspring | vertex (progeny per day) | heat stress resistance | oxidative stress resistance | autofluorescence (age pigment / lipofuscin) | ROS level | triglyceride / fat / lipid content | activity / quantity / expression of SODs | activity / quantity / expression of GSTs | mean lifespan | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
0.001 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 15°C | up | 9.67 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.01 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 15°C | up | 23.13 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 15°C | up | 4.21 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.001 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | up | 11.09 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.01 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | down | yes | A10; blue fluorescence | up | 16.09 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | up | 5.87 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.001 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | up | 23.45 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.01 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | up | 28.19 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 25°C | up | 10.39 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.01 µg/ml | whole life; start: L4 | OP50 | NGM agar | N2 (wild type) | 20°C | up | yes | A5; attractant: 1 M sodium acetate | up | yes | L4; stressor: 1 mM aldicarb; endpoint: ratio of non-paralyzed and paralyzed worms | down | yes | A5 and A10 | none | no | none | no | up | 15.09 | yes | A2; stressor: 35°C for 4 h; endpoint: survival | up | 50.00 | yes | A4; stressor: 250 µM juglone for 24 h; endpoint: survival | down | yes | A4; exposure to 37°C during measurement; measured via H2-DCF-DA | down | yes | A10 | up-regulation of age-1, pdk-1, daf-2, daf-16, skn-1, sod-3, and gst-4 | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | TK22 [mev-1(kn1)] | 20°C | up | 13.13 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | DA1116 [eat-2(ad1116)] | 20°C | up | 1.77 | no | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | PS3551 [hsf-1(sy441)] | 20°C | up | 2.33 | no | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | GR1307 [daf-16(mgDf50)] | 20°C | down | -1.14 | no | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | EU1 [skn-1(zu67)/nT1 [unc-?(n754) let-?]] | 20°C | up | 3.63 | no | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | CB1370 [daf-2(e1370)] | 20°C | up | 1.71 | no | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | up | 34.29 | yes | GST-4 expression; measured by fluorescence | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | up | 23.62 | yes | sod-3 expression; measured via fluorescence | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
0.1 µg/ml | whole life; start: L4 | OP50 | NGM agar | FUDR | CL4176 {smg-1(cc546); dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)]} | 15°C | down | yes | A2; pre-exposure to 25°C; endpoint: ratio of paralyzed and non-paralyzed worms | up | 17.62 | yes | Rathor L, Pant A, Awasthi H, Mani D, Pandey R (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18, 131-147. | no |