| Treatment | Caffeic acid phenethylester | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CID (PubChem) | 5281787 | ChEMBL | heat stress resistance | ROS level | activity / quantity / expression of CTLs | translocation of SKN-1 to nucleus | translocation of DAF-16 to nucleus | median lifespan | maximum lifespan | |||||||||||||||||||||||||||||||
| Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | ||||||
| 100 µM | whole life; start: L4/A1 | OP50-1 | liquid NGM | streptomycin; BSA | N2 (wild type) | 20°C | up | yes | A3; stressor: 37°C; endpoint: mean lifespan | down | -50.00 | yes | A3; pre-exposure to 37°C; measured via H2DCF-DA | down | -5.13 | no | A3 | DAF-16 | Havermann S, Chovolou Y, Humpf HU, Wätjen W (2014) Caffeic acid phenethylester increases stress resistance and enhances lifespan in Caenorhabditis elegans by modulation of the insulin-like DAF-16 signalling pathway. PLoS One 9, e100256. | |||||||||||||||||||||
| 100 µM | 1 h; start: L4/A1 | OP50-1 | liquid NGM | streptomycin; BSA | LD001 {ldIs007 [skn-1p::SKN-1::gfp]; pRF4 [rol-6(su1006gf)]} | 20°C | up | 28.57 | no | Havermann S, Chovolou Y, Humpf HU, Wätjen W (2014) Caffeic acid phenethylester increases stress resistance and enhances lifespan in Caenorhabditis elegans by modulation of the insulin-like DAF-16 signalling pathway. PLoS One 9, e100256. | ||||||||||||||||||||||||||||||
| 100 µM | 1 h; start: L4/A1 | OP50-1 | liquid NGM | streptomycin; BSA | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | up | 218.18 | yes | Havermann S, Chovolou Y, Humpf HU, Wätjen W (2014) Caffeic acid phenethylester increases stress resistance and enhances lifespan in Caenorhabditis elegans by modulation of the insulin-like DAF-16 signalling pathway. PLoS One 9, e100256. | ||||||||||||||||||||||||||||||
| 100 µM | whole life; start: L4/A1 | OP50-1 | liquid NGM | streptomycin; BSA | CF1038 [daf-16(mu86)] | 20°C | none | no | A3; stressor: 37°C; endpoint: mean lifespan | none | no | A3; pre-exposure to 37°C; measured via H2DCF-DA | Havermann S, Chovolou Y, Humpf HU, Wätjen W (2014) Caffeic acid phenethylester increases stress resistance and enhances lifespan in Caenorhabditis elegans by modulation of the insulin-like DAF-16 signalling pathway. PLoS One 9, e100256. | |||||||||||||||||||||||||||
| 100 µM | whole life; start: L4/A1 | HT115 (DE3) with RNAi-vector against skn-1 | liquid NGM | tetracycline; ampicillin; IPTG; BSA | N2 (wild type) | 20°C | none | no | A3; stressor: 37°C; endpoint: mean lifespan | Havermann S, Chovolou Y, Humpf HU, Wätjen W (2014) Caffeic acid phenethylester increases stress resistance and enhances lifespan in Caenorhabditis elegans by modulation of the insulin-like DAF-16 signalling pathway. PLoS One 9, e100256. | ||||||||||||||||||||||||||||||
| 100 µM | whole life; start: L4/A1 | OP50-1 | liquid NGM | streptomycin; BSA | N2 (wild type) | 25°C | up | 9.00 | yes | up | 17.00 | yes | Havermann S, Chovolou Y, Humpf HU, Wätjen W (2014) Caffeic acid phenethylester increases stress resistance and enhances lifespan in Caenorhabditis elegans by modulation of the insulin-like DAF-16 signalling pathway. PLoS One 9, e100256. | |||||||||||||||||||||||||||
| 100 µM | whole life; start: L4/A1 | OP50-1 | liquid NGM | streptomycin; BSA | CF1038 [daf-16(mu86)] | 25°C | down | -11.00 | yes | Havermann S, Chovolou Y, Humpf HU, Wätjen W (2014) Caffeic acid phenethylester increases stress resistance and enhances lifespan in Caenorhabditis elegans by modulation of the insulin-like DAF-16 signalling pathway. PLoS One 9, e100256. | ||||||||||||||||||||||||||||||
| 100 µM | whole life; start: L4/A1 | HT115 (DE3) with RNAi-vector against skn-1 | liquid NGM | tetracycline; ampicillin; IPTG; BSA | N2 (wild type) | 25°C | up | yes | Havermann S, Chovolou Y, Humpf HU, Wätjen W (2014) Caffeic acid phenethylester increases stress resistance and enhances lifespan in Caenorhabditis elegans by modulation of the insulin-like DAF-16 signalling pathway. PLoS One 9, e100256. | |||||||||||||||||||||||||||||||
| 100 µM | whole life; start: L4/A1 | HT115 (DE3) with RNAi-vector against skn-1 | liquid NGM | tetracycline; ampicillin; IPTG; BSA | CF1038 [daf-16(mu86)] | 25°C | down | yes | Havermann S, Chovolou Y, Humpf HU, Wätjen W (2014) Caffeic acid phenethylester increases stress resistance and enhances lifespan in Caenorhabditis elegans by modulation of the insulin-like DAF-16 signalling pathway. PLoS One 9, e100256. | |||||||||||||||||||||||||||||||