Treatment | Catechinic acid | physical function | reproductive function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | locomotion on solid media | | | | number of offspring | | | | speed of larval development | | | | quantity of early reproduction | | | | quantity of late reproduction | | | | activity / quantity / expression of HSPs | | | | ATP level | | | | mitochondrial content/quality | | | | maximum lifespan | | | | median lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | | | | | | |
1 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | n.d. | | up | 3.4 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
50 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | n.d. | | up | 3.3 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | OP50 | NGM agar | FUdR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | n.d. | | up | 41.9 | yes | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | | yes | A10; endpoints: speed of movement and body bends; no change at L4 and A5 | up | | yes | | none | | no | | none | | no | | up | | yes | | | | | | variable | | yes | decreased at A1, increased at A8 | down | | yes | A1; no significant change at A8; endpoint: ratio of mitochondrial to nuclear DNA | | | | | | | | | upregulation of pink-1, sqst-1, bec-1, vps-34, lgg-1 at A1, but unchanged expression at A8; downregulation of atp-1, mev-1 at A1, but upregulation of atp-1, mev-1, sdha-1 at A8; downregulation of fzo-1 at A1; | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against eat-2 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 22.1 | yes | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against daf-16 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 15.4 | yes | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against aak-2 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 6.7 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against mev-1 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 3.3 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against bec-1 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 3.1 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against vps-34 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 6.25 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against pink-1 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 3.7 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against dct-1 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 6.5 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against sqst-1 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 3 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against skn-1 | NGM agar | Ampicillin, IPTG, FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 5.5 | no | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | OP50 | NGM agar | | SJ4103 {zcIs14 [myo-3::GFP(mit)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A1; no significant change at A8; endpoints: mitochondrial content in gut and muscles | | | | | | | | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | OP50 | NGM agar | | SJ4143 {zcIs17 [ges-1::GFP(mit)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A1; no significant change at A8; endpoints: mitochondrial content in gut and muscles | | | | | | | | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | OP50 | NGM agar | | SJ4100 {zcls13 [hsp-6::GFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | down | | no | A1 | | | | | | | | | | | | | | | | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against bec-1 | NGM agar | Ampicillin, IPTG | SJ4103 {zcIs14 [myo-3::GFP(mit)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | A1;endpoints: mitochondrial content in muscles | | | | | | | | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against pink-1 | NGM agar | Ampicillin, IPTG | SJ4103 {zcIs14 [myo-3::GFP(mit)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | down | | no | A1;endpoints: mitochondrial content in muscles | | | | | | | | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against bec-1 | NGM agar | Ampicillin, IPTG | SJ4143 {zcIs17 [ges-1::GFP(mit)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | no | A1;endpoints: mitochondrial content in gut | | | | | | | | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |
200 µM | whole life; start: L1 | HT115 (DE3) with RNAi-vector against pink-1 | NGM agar | Ampicillin, IPTG | SJ4143 {zcIs17 [ges-1::GFP(mit)]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | | no | A1;endpoints: mitochondrial content in gut | | | | | | | | | | | | | Wu X, Al-Amin M, Zhao C, An F, Wang Y, Huang Q, Teng H, Song H (2020) Catechinic acid, a natural polyphenol compound, extends the lifespan of Caenorhabditis elegans via mitophagy pathways. Food Funct 11, 5621-5634. | no |