Treatment | Endopleura uchi, water extract | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | pharyngeal pumping | | | | body length / growth rate | | | | oxidative stress resistance | | | | ROS level | | | | activity / quantity / expression of HSPs | | | | activity / quantity / expression of SODs | | | | quantity of polyQ aggregates | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | | | | | | |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | up | | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | up | | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
200 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | N2 (wild type) | 20°C | | | | | | | | | up | 185.19 | yes | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | down | -80 | yes | A1; measured via H2DCF-DA | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
300 µg/ml | whole life; start: A1 | OP50 | NGM agar | | N2 (wild type) | 20°C | up | 128 | yes | A10; similar change at A5 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
300 µg/ml | whole life; start: L4 | OP50 | n.d. | | N2 (wild type) | 20°C | | | | | up | 6.2 | no | A1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
300 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | BA17 [fem-1(hc17)] | 25°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | up | 33 | yes | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | down | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
200 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | down | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
300 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1038 [daf-16(mu86)] | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | | | | | none | | no | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | GR1307 [daf-16(mgDf50)] | 20°C | | | | | | | | | none | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | GR1307 [daf-16(mgDf50)] | 20°C | | | | | | | | | down | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
200 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | GR1307 [daf-16(mgDf50)] | 20°C | | | | | | | | | down | | no | A2; stressor: 80 µM juglone for 24h starting at A1; endpoint: survival rate | | | | | | | | | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | up | 44 | yes | A1 | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | up | | yes | A1 | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
200 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | | | | | up | | yes | A1 | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
50 µg/ml | whole life; start: L4 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | down | | yes | A4; pre-exposed to juglone | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
100 µg/ml | whole life; start: L4 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | down | | yes | A4; pre-exposed to juglone | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
200 µg/ml | whole life; start: L4 | OP50 | liquid S-medium | | TJ375 {gpIs1 [hsp-16.2p::GFP]} | 20°C | | | | | | | | | | | | | | | | | down | | yes | A4; pre-exposed to juglone | | | | | | | | | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
50 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | AM141 {rmIs133 [unc-54p::Q40::YFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | down | | no | A1 | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
100 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | AM141 {rmIs133 [unc-54p::Q40::YFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | down | | yes | A1 | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |
200 µg/ml | whole life; start: L1 | OP50 | liquid S-medium | | AM141 {rmIs133 [unc-54p::Q40::YFP]} | 20°C | | | | | | | | | | | | | | | | | | | | | | | | | down | -60 | yes | A1 | | | | | | | | | Peixoto H, Roxo M, Silva E, Valente K, Braun M, Wang X, Wink M (2019) Bark Extract of the Amazonian Tree Endopleura uchi (Humiriaceae) Extends Lifespan and Enhances Stress Resistance in Caenorhabditis elegans. Molecules 24, 915. | no |