Treatment | Polydatin | physical function | physiological function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | 5281718 | ChEMBL | | | | | locomotion in liquid media | | | | oxidative stress resistance | | | | activity / quantity / expression of SODs | | | | activity / quantity / expression of GSTs | | | | translocation of DAF-16 to nucleus | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | Change compared to control | Percentual change compared to control | p < 0.05 | notes | | | | | | |
0.01 mM | 24 h; start: L1 | OP50 | liquid K-medium | | N2 (wild type) | 20°C | | | | | up | 17.05 | yes | young adults; stressor: 500 µMCu2+ for 24 h; endpoint: mean lifespan | | | | | | | | | | | | | up | 11.19 | yes | | up-regulation of daf-16, daf-2, and sir-2.1; down-regulation of skn-1 | | | daf-2, daf-16, sod-3 | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |
0.1 mM | 24 h; start: L1 | OP50 | liquid K-medium | | N2 (wild type) | 20°C | | | | | up | 26.61 | no | young adults; stressor: 500 µMCu2+ for 24 h; endpoint: mean lifespan | | | | | | | | | | | | | up | 17.48 | yes | | up-regulation of daf-16; down-regulation of daf-2, sir-2.1, and skn-1 | | | daf-2, daf-16, sod-3 | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |
1 mM | 24 h; start: L1 | OP50 | liquid K-medium | | N2 (wild type) | 20°C | up | | yes | A1, A3, and A5; significant change in A3 and A5 after pre-exposure to Cu2+ and in A3 without pre-exposure | up | 61.50 | yes | young adults; stressor: 500 µMCu2+ for 24 h; endpoint: mean lifespan | | | | | | | | | | | | | up | 30.07 | no | | up-regulation of daf-16; down-regulation of daf-2 | | | daf-2, daf-16, sod-3 | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |
1 mM | 24 h; start: L1 | OP50 | liquid K-medium | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | down | | yes | A1, A3, and A5; pre-exposed to Cu2+; similar change without Cu2+ in A3 and A5, but unchanged in A1; measured by fluorescence | | | | | | | | | | | | | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |
0.01 mM | 24 h; start: L1 | OP50 | liquid K-medium | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | up | | yes | A3; no significant changes in A1 and A5 | | | | | | | | | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |
0.1 mM | 24 h; start: L1 | OP50 | liquid K-medium | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | up | | yes | A3; no significant changes in A1 and A5 | | | | | | | | | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |
1 mM | 24 h; start: L1 | OP50 | liquid K-medium | | TJ356 {zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | | | | | | | | | up | | yes | A1, A3, and A5 | | | | | | | | | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |
0.01 mM | 24 h; start: L1 | OP50 | liquid K-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | up | | no | A1, A3, and A5; measured by fluorescence | | | | | | | | | | | | | | | | | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |
0.1 mM | 24 h; start: L1 | OP50 | liquid K-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | up | | no | A1, A3, and A5; measured by fluorescence | | | | | | | | | | | | | | | | | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |
1 mM | 24 h; start: L1 | OP50 | liquid K-medium | | CF1553 {muIs84 [(pAD76)sod-3p::GFP + rol-6(su1006)]} | 20°C | | | | | | | | | up | | yes | A1, A3, and A5; measured by fluorescence | | | | | | | | | | | | | | | | | Wen H, Gao X, Qin J (2014) Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip. Integr Biol (Camb) 6, 35-43. | |