Treatment | phenformin | physical function | reproductive function | healthspan biomarkers | lifespan | Transcriptome | Proteome | Metabolome | Target | Reference | blinded |
CID (PubChem) | | ChEMBL | | | | | body length / growth rate | | | | number of offspring | | | | vertex (progeny per day) | | | | activity / quantity / expression of GSTs | | | | mean lifespan | | | | | | | | | |
Concentration | Exposure time | Food | Growth medium | Special additives | C. elegans strain | Temperature | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | change compared to control | percentage change compared to control | significant (p < 0.05) | notes | | | | | | |
1.5 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | up | 5.35 | yes | | A2; up-regulation of pAAK-2 | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
3 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | up | 20.66 | yes | | A2; up-regulation of pAAK-2 | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
4.5 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | N2 (wild type) | 20°C | | | | | | | | | | | | | | | | | up | 26.38 | yes | | A2; up-regulation of pAAK-2 | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
4.5 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | GA1001 [aak-2(ok524)] | 20°C | | | | | | | | | | | | | | | | | down | -15.48 | yes | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
4.5 mM | whole life; start: L4 | OP50 | NGM agar | FUDR | EU31 [skn-1(zu135)/nT1 [unc-?(n754) let-?]] | 20°C | | | | | | | | | | | | | | | | | down | -3.21 | no | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
4.5 mM | whole life; start: L1 | OP50 | NGM agar | | CL2166 {dvIs19 [(pAF15)gst-4p::GFP::NLS]} | 20°C | | | | | | | | | | | | | up | | yes | L4; expression of gst-4; measured via fluorescence | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
4.5 mM | whole life; start: L1 | OP50 | NGM agar | | GA1112 {aak-2(ok524); dvIs19 [pAF15 (gst-4::GFP::NLS)]} | 20°C | | | | | | | | | | | | | none | | no | L4; expression of gst-4; measured via fluorescence | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
1.5 mM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | down | | yes | L4 | down | | no | | down | | yes | day 2 | | | | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
3 mM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | down | | yes | L4 | down | | yes | | down | | yes | day 1-3 | | | | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |
4.5 mM | whole life; start: L1 | OP50 | NGM agar | | N2 (wild type) | 20°C | down | | yes | L4 | down | | yes | | down | | yes | day 1-3 | | | | | | | | | | | | | Cabreiro F, Au C, Leung KY, Vergara-Irigaray N, Cochemé HM, Noori T, Weinkove D, Schuster E, Greene ND, Gems D (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153, 228-239. | no |